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Catalog # HM2061F

MBL, Human, mAb 3E7, FITC

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Mannose Binding Lectin (MBL) also called mannose- or mannan-binding protein (MBP) is a member of the group of collectins. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. It forms a complex with C1r/C1s like serine proteases designated MASPs that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q-independent. MBL exhibits complement-dependent antibacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver or serum of various vertebrate species.

Application
Flow cytometry, Frozen sections, Functional studies, Immuno assays

Application Notes
FC: cells were incubated with 1.0 μg/ml MBL in PBS containing 1%BSA (w/v), 0.01% NaN3 (w/v) and 10 mM CaCl2 for 30 min at 0°C. The cells were washed with the same buffer, and incubated with antibody clone 3E7, 6.5 μg/ml. After 30 min at 0°C, cells were washed and incubated with phycoerythrin (PE)-conjugated goat F(ab’)2 anti-mouse Ig (Ref.3).
IHC-F: Tissue sections embedded in ornithine carbamoyltransferase compound were frozen in acetone dry ice and immersed in Tris-buffered saline (TBS) three times for 5 minutes. Treatment in 0.5% hydrogen peroxide in methanol solution has been done to quench endogenous peroxidase activity (Ref.2).
FS: Pretreatment of sheep erythrocytes with antibody 3E7 suppressed hemolysis in a dose dependent manner (Ref.1).

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Negative Control
Nonimmune normal serum or TBS

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
Conjugate
FITC
Immunogen
Serum isolated human RaRF
Isotype
Mouse IgG1
Species
Human
Alias
Mannan Binding Lectin, mannose- or mannan-binding protein, MBP
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Infectious diseases
Protocol
Protocol Western Blot
59 kb
Protocol SDS Page
57 kb
Protocol Sandwich ELISA
49 kb
Protocol Flow Cytometry
58 kb
Protocol IHC-F
36 kb
CoA-TDS HM1067-HM2099
HM2061F-100UG
125 kb
HM2061F-20UG
125 kb

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