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Catalog # HM1040F

MD-1, Mouse, mAb MD113, FITC

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The monoclonal antibody MD113 reacts with mouse MD-1. MD-1 (22-25 kD) is an extracellular protein associated with the extracellular domain of RP105. The latter is a type 1 transmembrane protein with leucine-rich repeats. RP105 is similar to Drosophila Toll. MD-1 associated with RP105 (similar to TLR4/MD-2) appears to be an essential molecule that helps RP105 on B-cells to signal to LPS. The MD113 antibody is antagonistic in LPS-induced B-cell proliferation and B7.2 up-regulation.

Application
Flow cytometry, Immuno fluorescence

Application Notes
The monoclonal antibody MD113 can be used for flow cytometry and functional studies. The monoclonal antibody cannot be used for Western blot and immunoprecipitation.

Use
For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
1 ml (100 µg/ml) 0.2 µm filtered FITC conjugated antibody solution in PBS, containing 1.0% bovine serum albumin and 0.02% sodium azide.
Conjugate
FITC
Isotype
Rat IgG2b
Species
Mouse
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Infectious diseases
Protocol
Protocol Flow Cytometry
58 kb
CoA-TDS HM1000-HM1066
HM1040F-100UG
47 kb
HM1040F-20UG
47 kb

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