Nectin (originally isolated as poliovirus receptor-related protein (PRR)) is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectin is colocalized with afadin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. The nectin family consist of nectin-1, nectin-2 and nectin-3. Nectin-2 also known as PRR2 or CD112 is a plasma membrane adhesion molecule localized at adherens junctions which is widely expressed in various cell lines including neuronal, endothelial, epithelial and hematopoietic cells. Next to its role in adherens junctions it functions as alphaherpus virus receptor and acts also as intercellular adhesion molecule and pseudorabies virus receptor. Disruption of mouse nectin-2 leads to infertility of male mice. Monoclonal antibody 502-57 has been raised against the extracellular domain of mouse nectin-2. The antibody cross reacts with human nectin-2.
Frozen sections, Immuno assays, Immuno fluorescence, Immuno precipitation, Western blot
F: Fixation with PFA or acetone/methanol is recommended.
IA: HM1052 can be used as a detection antibody.
W: proteins were separated by SDS-PAGE and electrophoretically transferred onto polyvinylidene fluoride sheets, which were then incubated with primary antibodies. Binding was detected with HRP-conjugated secondary antibodies and Western blotting detection reagents (Ref.3).
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.