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Catalog # HM1052F

Nectin-2, Mouse, mAb 502-57, FITC

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Nectin (originally isolated as poliovirus receptor-related protein (PRR)) is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectin is colocalized with afadin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. The nectin family consist of nectin-1, nectin-2 and nectin-3. Nectin-2 also known as PRR2 or CD112 is a plasma membrane adhesion molecule localized at adherens junctions which is widely expressed in various cell lines including neuronal, endothelial, epithelial and hematopoietic cells. Next to its role in adherens junctions it functions as alphaherpus virus receptor and acts also as intercellular adhesion molecule and pseudorabies virus receptor. Disruption of mouse nectin-2 leads to infertility of male mice. Monoclonal antibody 502-57 has been raised against the extracellular domain of mouse nectin-2. The antibody cross reacts with human nectin-2.

Application
Frozen sections, Immuno assays, Immuno fluorescence, Immuno precipitation, Western blot

Application Notes
F: Fixation with PFA or acetone/methanol is recommended.
IA: HM1052 can be used as a detection antibody.
W: proteins were separated by SDS-PAGE and electrophoretically transferred onto polyvinylidene fluoride sheets, which were then incubated with primary antibodies. Binding was detected with HRP-conjugated secondary antibodies and Western blotting detection reagents (Ref.3).

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 μg/ml) 0.2 μm protein G purified FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
Conjugate
FITC
Isotype
Rat IgG2a
Species
Mouse
Cross reactivity
Human – Yes
Alias
Herpes virus entry mediator B, Herpesvirus entry mediator B, HveB, Murine herpes virus entry protein B, mHveB, Nectin cell adhesion molecule 2, Poliovirus receptor homolog, Poliovirus receptor-related protein 2, CD112<br> - Gene name: Nectin2, Mph, Pvr, Pvrl2, Pvs
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Infectious diseases
Protocol
Protocol Western Blot
59 kb
Protocol SDS Page
57 kb
Protocol Sandwich ELISA
49 kb
Protocol Immuno precipitation
54 kb
Protocol Flow Cytometry
58 kb
Protocol IHC-F
36 kb
CoA-TDS HM1000-HM1066
HM1052F-100UG
78 kb
Assay Manual
HM1052F-20UG
78 kb

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