Home \ Antibodies \ SPLUNC1, Mouse, pAb
Catalog # HP8043

SPLUNC1, Mouse, pAb

120,00462,00

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The polyclonal antibody recognizes mouse SPLUNC1 (Short palate, lung and nasal epithelium clone 1). SPLUNC1, also known as known as BPI fold-containing family member A1 (BPIFA1), is an abundant protein present in the airway lining fluid of healthy humans and mice. It is mainly produced by epithelial cells in the lung and tracheobronchial region, the submucosal glands of the trachea, the bronchi, and in specific granules of neutrophils. The protein displays antibacterial activity against Gram-negative bacteria such as P. aeruginosa and is thought to be involved in inflammatory responses to irritants in the upper airways. Additionally, it may also serve as a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer. Its expression is modulated in multiple lung diseases including cystic fibrosis, COPD, respiratory malignancies, and idiopathic pulmonary fibrosis. However, the precise role of SPLUNC1 in pulmonary pathogenesis remains to be elucidated.

Application
Paraffin sections, Western blot

Application Notes
W: A reduced sample treatment and SDS-Page was used. The band size is 50 kDa (Ref.2). IHC-P: Tissue sections were pretreated with tri-sodium citrate buffer for 8 minutes in a microwave oven followed by rinsing in PBS. Tissue sections were fixed in 3 % H2O2 in methanol for 20 minutes (Ref.1)

Use
For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Positive Control
Nasopharyngeal tissues

Product type
Polyclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen
Generated against epitope 31-46 (GPPLPLNQGPPLPLNQ)
Isotype
Rabbit Ig
Species
Mouse
Cross reactivity
Rabbit – Yes
Alias
BPI fold-containing family member A1, BPIFA1
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least - one year. The exact expiry date is indicated on the label.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Pulmonology, Tumor immunology
Protocol CoA-TDS pAb
HP8043
123 kb

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