The polyclonal antibody recognizes mouse SPLUNC1 (Short palate, lung and nasal epithelium clone 1). SPLUNC1, also known as known as BPI fold-containing family member A1 (BPIFA1), is an abundant protein present in the airway lining fluid of healthy humans and mice. It is mainly produced by epithelial cells in the lung and tracheobronchial region, the submucosal glands of the trachea, the bronchi, and in specific granules of neutrophils. The protein displays antibacterial activity against Gram-negative bacteria such as P. aeruginosa and is thought to be involved in inflammatory responses to irritants in the upper airways. Additionally, it may also serve as a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer. Its expression is modulated in multiple lung diseases including cystic fibrosis, COPD, respiratory malignancies, and idiopathic pulmonary fibrosis. However, the precise role of SPLUNC1 in pulmonary pathogenesis remains to be elucidated.
Paraffin sections, Western blot
W: A reduced sample treatment and SDS-Page was used. The band size is 50 kDa (Ref.2).
IHC-P: Tissue sections were pretreated with tri-sodium citrate buffer for 8 minutes in a microwave oven
followed by rinsing in PBS. Tissue sections were fixed in 3 % H2O2 in methanol for 20 minutes (Ref.1)
For Western blotting, dilutions to be used depend on detection system applied. It is recommended that
users test the reagent and determine their own optimal dilutions. The typical starting working dilution is
1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.