Home \ Antibodies \ TIM-4, Mouse, mAb Kat5-18, FITC
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Catalog # HM1111F

TIM-4, Mouse, mAb Kat5-18, FITC

120,00314,00

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The monoclonal antibody Kat5-18 recognizes T-cell immunoglobulin and mucin domain containing molecule (TIMD-4, also called TIM-4). TIMD-4 is a type I transmembrane protein belonging to the immunoglobulin superfamily, containing one Ig-like V-type (immunoglobulin-like) domain. TIMD-4 is expressed in Mac1+ cells in spleen, lymph nodes and fetal liver. TIMD-4 binds apoptotic cells by recognizing phosphatidylserine via its immunoglobulin domain. TIMD-4 in fibroblasts enhanced their ability to engulf apoptotic cells. TIMD-4 is involved in regulating T-cell proliferation and lymphotoxin signaling. TIMD-4 and TIMD-1 are phosphatidylserine receptors for the engulfment of apoptotic cells, and are also involved in intracellular signaling in which exosomes are involved.
The monoclonal antibody Kat5-18 blocks the engulfment of apoptotic cells by thymic macrophages.

Application
Flow cytometry, Functional studies, Immuno assays, Immuno fluorescence

Application Notes
FC: Cells were incubated on ice for 30 min with 1 μg/ml biotinylated Kat5-18 in 100 μl staining solution (PBS containing 2% FCS), followed by incubation with 2 μg/ml Allophycocyanin (APC)-Cyanine 7 (Cy7)-conjugated streptavidin and 2 μg/ml APCconjugated anti-CD11b. The cells were then stained with 0.5 μM SYTOX Blue (Ref.2).
FS: Antibody Kat5-18 blocks the engulfment of apoptotic cells by thymic macrophages (Ref.1).

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 ml (100 μg/ml) 0.2 μm protein A purified FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
Conjugate
FITC
Immunogen
Mouse peritoneal cells (Ref.1)
Isotype
Armenian hamster IgG
Species
Mouse
Alias
T-cell immunoglobulin and mucin domain-containing protein 4, TIMD-4, Spleen, mucin-containing, knockout of lymphotoxin protein, SMUCKLER, T-cell immunoglobulin mucin receptor 4, TIM-4, T-cell membrane protein 4</br> - Gene name: Timd4, Tim4
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Autoimmunity, Tumor immunology
CoA-TDS HM1067-HM2099

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