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Catalog # HM1111

TIM-4, Mouse, mAb Kat5-18

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The monoclonal antibody Kat5-18 recognizes T-cell immunoglobulin and mucin domain containing molecule (TIMD-4, also called TIM-4). TIMD-4 is a type I transmembrane protein belonging to the immunoglobulin superfamily, containing one Ig-like V-type (immunoglobulin-like) domain. TIMD-4 is expressed in Mac1+ cells in spleen, lymph nodes and fetal liver. TIMD-4 binds apoptotic cells by recognizing phosphatidylserine via its immunoglobulin domain. TIMD-4 in fibroblasts enhanced their ability to engulf apoptotic cells. TIMD-4 is involved in regulating T-cell proliferation and lymphotoxin signaling. TIMD-4 and TIMD-1 are phosphatidylserine receptors for the engulfment of apoptotic cells, and are also involved in intracellular signaling in which exosomes are involved.
The monoclonal antibody Kat5-18 blocks the engulfment of apoptotic cells by thymic macrophages.

Application
Flow cytometry, Functional studies, Immuno assays, Immuno fluorescence

Application Notes
FC: Cells were incubated on ice for 30 min with 1 μg/ml biotinylated Kat5-18 in 100 μl staining solution (PBS containing 2% FCS), followed by incubation with 2 μg/ml Allophycocyanin (APC)-Cyanine 7 (Cy7)-conjugated streptavidin and 2 μg/ml APCconjugated anti-CD11b. The cells were then stained with 0.5 μM SYTOX Blue (Ref.2).
FS: Antibody Kat5-18 blocks the engulfment of apoptotic cells by thymic macrophages (Ref.1).

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg, 500 µg
Formulation
Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.
Immunogen
Mouse peritoneal cells (Ref.1)
Isotype
Armenian hamster IgG
Species
Mouse
Alias
T-cell immunoglobulin and mucin domain-containing protein 4, TIMD-4, Spleen, mucin-containing, knockout of lymphotoxin protein, SMUCKLER, T-cell immunoglobulin mucin receptor 4, TIM-4, T-cell membrane protein 4</br> - Gene name: Timd4, Tim4
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Autoimmunity, Tumor immunology
Protocol
Protocol Sandwich ELISA
49 kb
Protocol Immuno Fluorescence
39 kb
Protocol Flow Cytometry
58 kb
CoA-TDS HM1067-HM2099
HM1111-100UG
79 kb
HM1111-20UG
99 kb
HM1111-500UG
79 kb
HM1111F-100UG
79 kb
HM1111F-20UG
99 kb
Safety Data Sheet
SDS - HM1111-100UG
51 kb
SDS - HM1111-20UG
51 kb
SDS - HM1111-500UG
50 kb

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