Home \ Antibodies \ TIMP-2, Human, pAb
Catalog # HP9076

TIMP-2, Human, pAb

This antibody recognizes tissue inhibitors of metallproteinase 2 (TIMP-2)

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Tissue inhibitors of metallproteinases (TIMPs) control metallo proteinases (MMPs), which are a large class of catalytic enzymes involved in a wide range of processes regulating tissue homeostasis. MMPs are formed as inactive zymogens which must be cleaved to become active. Activated MMPs are inhibited by four TIMPs. TIMPs form non-covalent complexes with MMPs. MMPs function through a divalent metal ion in their catalytic domain. TIMPS bind to this catalytic domain in a 1:1 ratio. TIMP and MMP expression and function are tightly regulated in order to contain a balance in proteolysis and proteolysis inhibition. Each TIMP targets multiple enzymes. TIMPs are ca 21Kda and share approximately 40% in amino acid sequence. The inhibitory capacity is located within the N-terminal domain. TIMP1 and TIMP3 are inducible, cell cycle regulated, glycosylated proteins expressed in many organs. TIMP2 is ubiquitous and constitutively expressed, whereas TIMP3 is tissue restricted. As extracellular proteins, the secretion, endocytosis and binding partners of TIMPs influence their localization. All TIMPs are secreted, but only TIMP3 is incorporated into the matrix. TIMP2 is associated with cancers, sclerosis and kidney injury. TIMP2 has a unique function given its crucial role in the formation of the MT1-MMP–TIMP2–proMMP2 ternary complex responsible for proMMP2 activation at the cell surface.

Application
Immuno assays, Immuno fluorescence, Western blot

Application Notes
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Polyclonal antibodies
Quantity
100 µg
Formulation
0.1 ml 0.2 μm filtered in PBS+0.02%NaN3+0.5%BSA+50%glycerol
Immunogen
Synthetic peptide, human TIMP-2
Isotype
Rabbit Ig
Species
Human
Cross reactivity
Mouse – Yes, Rat – Yes
Storage and stability
Product should be stored at -20°C. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
CoA-TDS pAb
HP9076
49 kb

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