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Catalog # HM1047F

TLR2, Mouse, mAb 6C2, FITC

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Monoclonal antibody 6C2 reacts with mouse Toll-like receptor 2 (TLR2, CD282). Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR2 has been identified as a receptor that is central to the innate immune response to lipoproteins of gram-negative bacteria, several whole gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. A functional interaction between TLR2 and TLR6 in the cellular response to various bacterial products has been discovered. The currently accepted paradigm regards TLR2 as an essential receptor for many eubacterial cell wall components, including lipoproteins and peptidoglycan. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2.

Flow cytometry, Frozen sections, Immuno fluorescence, Immuno precipitation

Application Notes
FC: Antibody 6C2 stains the intracellular domain of mouse TLR2. For intracellular staining cells were permeabilized with buffer containing 0.5% saponin in PBS-1% FCS. The cells were fixed in 2% paraformaldehyde before staining. (Ref.1)
IF: RAW cells were fixed in in 1% paraformaldehyde/PBS for 30 minutes at R.T. and blocked with PBS/3% BSA solution for 30 minutes. HM1047 was incubated overnight.
IHC-F: Frozen kidney sections were dried by airflow, fixed in cold acetone and blocked with 0.09% H2O2 in PBS.

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 ml (100 µg/ml) 0.2 µm protein G purified FITC conjugated filtered antibody solution in PBS, containing 1.0% bovine serum albumin and 0.02% sodium azide.
CHO/moTLR2 cells (Ref.1)
Rat IgG2b
Cross reactivity
Human – No
Toll-like Receptor 2, CD282 <br /> - Gene name: Tlr2
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Infectious diseases
Protocol CoA-TDS HM1000-HM1066

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