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TNF-alpha, Human, mAb T1, FITC-0
Catalog # HM2024F

TNF-alpha, Human, mAb T1, FITC

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The antibody reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. The antibody inhibits the biological activity of both forms. It does not react with receptor bound TNF-alpha. It can be a useful tool to discriminate between the membrane form of TNF expressed on producer cells and the proteolytically cleaved, soluble TNF-alpha bound to its cognate cell membrane receptors (TNF-RI and TNF-RII). For this purpose we recommend to use this antibody in combination with the anti-TNF-alpha antibody HM2026, which recognizes soluble, membrane and receptor bound TNF-alpha.

Application
Flow cytometry, Functional studies, Immuno assays, Western blot

Application Notes
FS: The monoclonal antibody T1 can be used for inhibition of the biological activity of TNF-alpha.
W: A reduced sample treatment and SDS-Page was used. The band size is 17 kDa.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 μg/ml) 0.2 μm protein G purified FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
Conjugate
FITC
Immunogen
BALB/c mice with huTNF, fusion of spleen cells with NSO myeloma cells (ref.1)
Isotype
Mouse IgG1
Species
Human
Alias
Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, C-domain 2, Tumor necrosis factor, soluble form<br> - Gene name: TNFA, TNFSF2
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
Disease
Autoimmunity, Gastroenterology
CoA-TDS HM1067-HM2099
HM2024F-20UG
89 kb

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