Home \ Antibodies \ YKL-40, Human, mAb mAY
Catalog # HM2293

YKL-40, Human, mAb mAY

120,00375,00

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Monoclonal antibody mAY recognizes human YKL-40. YKL-40 is a human glycoprotein which is related to the chitinase protein family. However, without chitinase activity. YKL-40 is also called chitinase-3-like protein 1 or BRP-39. YKL-40 is expressed and secreted by inflammatory cells and some cancer cells.YKL-40 has no distinct function but is involved in processes related to inflammation, extracellular matrix (ECM) remodelling and proliferation of cancer cells. The cellular receptor is still unknown. Protein expression is predominantly found in macrophages, neutrophils and differentiated vascular smooth muscle cells, especially in tissues with inflammation vascular damage and ECM remodelling. Furthermore, YKL-40 stimulates the proliferation of connective tissue cells, like fibroblasts, in a dose dependent way. Studies report that YKL-40 signalling is associated with MAPK and PI3K pathways and possibly counteracts TNFα and Il1. YKL-40 is related to several pathogenic processes like infection, cardiovascular disease, fibrosis, diabetes, osteoarthritis and various cancers. Several studies demonstrate elevated levels in those diseases and the protein is investigated to function as a clinical biomarker. Serum YKL-40 is found to be a biomarker of prognosis already in 13 different types of cancer and associated with disease severity. In healthy individuals it is believed that there is no difference between gender and the serum concentration is quite constant.
Antibody mAY is capable of binding native as well as recombinant YKL-40 and is capable to inhibit tube formation of microvascular endothelial cells in vitro. The antibody also blocks MAPK signalling via VEGFR2.

Application
Functional studies, Immuno fluorescence, Immuno precipitation, Western blot

Application Notes
W: A reduced sample treatment and SDS-Page was used. The band size (s) is 40 kDa (Ref.1). FS: Antibody mAY functions as neutralisation antibody. The antibody was functionally tested by cell viability assay and tumor xenografts. The biological activity of the antibody can be defined as neutralizing activity on angiogenesis in vitro and in vivo (Ref.1)

Use
For Western blotting and Immuno Precipitation, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Immunogen
Recombinant full-length human YKL-40
Isotype
Mouse IgG1
Species
Human
Alias
chitinase-3-like protein 1, BRP-39
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product
Disease
Cardiology and metabolism, Tumor immunology
CoA-TDS HM2200 - 2300
HM2293
80 kb

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