Nectin-2, Mouse, mAb 502-57, FITC

Catalog #: HM1052F
Quantity: 100 µg
$574.00

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Description
Details
Nectin (originally isolated as poliovirus receptor-related protein (PRR)) is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectin is colocalized with afadin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. The nectin family consist of nectin-1, nectin-2 and nectin-3. Nectin-2 also known as PRR2 or CD112 is a plasma membrane adhesion molecule localized at adherens junctions which is widely expressed in various cell lines including neuronal, endothelial, epithelial and hematopoietic cells. Next to its role in adherens junctions it functions as alphaherpus virus receptor and acts also as intercellular adhesion molecule and pseudorabies virus receptor. Disruption of mouse nectin-2 leads to infertility of male mice. Monoclonal antibody 502-57 has been raised against the extracellular domain of mouse nectin-2. The antibody cross reacts with human nectin-2.
Specifications

Specifications

Catalog number HM1052F
Product type Monoclonal antibodies
Quantity 100 µg
Formulation 1 ml (100 μg/ml) 0.2 μm protein G purified FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
Conjugate FITC
Isotype Rat IgG2a
Species Mouse
Cross reactivity Human - Yes
Alias Herpes virus entry mediator B, Herpesvirus entry mediator B, HveB, Murine herpes virus entry protein B, mHveB, Nectin cell adhesion molecule 2, Poliovirus receptor homolog, Poliovirus receptor-related protein 2, CD112
Gene name: Nectin2, Mph, Pvr, Pvrl2, Pvs
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Infectious diseases
Application
Applications
  • Application:
    F, IA, IF, IP, W
  • Application Notes:
    F: Fixation with PFA or acetone/methanol is recommended.
    IA: HM1052 can be used as a detection antibody.
    W: proteins were separated by SDS-PAGE and electrophoretically transferred onto polyvinylidene fluoride sheets, which were then incubated with primary antibodies. Binding was detected with HRP-conjugated secondary antibodies and Western blotting detection reagents (Ref.3).
  • Use:
    Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
References
References
References:
1. Takahashi, K et al; Nectin/PRR: An immunoglobulin-like cell adhesion molecule recruited to cadherin-based adherens junctions through interaction with afadin, a PDZ domain-containing protein. J Cell Biol 1999, 145: 539
2. Kawabe, H et al; Pilt, a novel peripheral membrane protein at tight junctions in epithelial cells. J Biol Chem 2001, 276: 48350
3. Sugimoto, M et al; The keratin-binding protein Albatross regulates polarization of epithelial cells. JCB 2008; 183: 19
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