L-Afadin, Rat, mAb 3

Catalog #: HM3013
Quantity: 100 µg
$474.00

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Description
Details
Afadin is an actin filament-binding protein that is involved in cell-cell adherens junctions (AJs) which directly interacts with the alpha herpus virus receptor, a member of the immunoglobulin supergene family. Afadin is colocalized with nectin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. L-Afadin has been isolated as an F-actin-binding protein from rat brain. L-afadin is ubiquitously expressed and specifically localized at zonula adherens (ZA) in epithelial cells and at cell-cell adherens junctions (AJs) in non-epithelial cells. L-afadin has a splice variant, named small intestine absorptive afadin (s-afadin) which is abundantly expressed in neural tissue. Monoclonal antibody 3 has been raised against peptide 1814-1829 aa of rat l-afadin. Monoclonal antibody 3 cross reacts with human, mouse and dog l-afadin.
Specifications

Specifications

Catalog number HM3013
Product type Monoclonal antibodies
Quantity 100 µg
Formulation 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype Mouse IgG1
Species Rat
Cross reactivity Canine - Yes, Human - Yes, Mouse - Yes
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Neurological disorders
Application
Applications
  • Application:
    F, IA, IP, W
  • Use:
    For Western blot and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Fixation with PFA or acetone/methanol is recommended.
References
References
References:
1. Sakisaka, T et al; Different behavior of l-afadin and neurabin-II during the formation and destruction of cell-cell adherens junction. Oncogene 1999, 18: 1609
2. Takahashi, K et al; Nectin/PRR: An immunoglobulin-like cell adhesion molecule recruited to cadherin-based adherens junctions through interaction with afadin, a PDZ domain-containing protein. J Cell Biol 1999, 145: 539
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