ASGPR, Rat, mAb 8D7

Catalog #: HM3020
Quantity: 100 µg
$474.00

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Description
Details
The asialoglycoprotein (ASGP) receptor is a transmembrane hepatocellular surface carbohydrate binding glycoproteins lacking terminal sialic acid residues (asialoglycoproteins). Characterization of the ASGP receptor- revealed its functional role in the binding, internalization and transport of a wide range of glycoproteins, which have exposed galactose or N-acetylgalactosamine residues, via the process of receptor-mediated endocytosis (RME). The ASGP receptor can bind a variety of important plasma proteins including transport proteins (i.e. transferrin), enzymes such as alkaline phosphatase, immunoglobulins including IgA, apoptotic hepatocytes, fibronectin and platelets. Additionally, the expression of the ASGP receptor has been clinically correlated to the level of hepatic function that is lost during liver diseases related to cancer, viral hepatitis, and cirrhosis. The ASGP receptor consists of major and minor subunits, which in the rat were identified as rat hepatic lectin (RHL) 1 and RHL 2/3, with molecular weights of respectively 42, 49 and 54 kDa. The selective binding (calcium and pH depended) and uptake of terminal galactosyl bearing proteins requires the formation of hetero-oligomers between these major and minor forms. The total ASGP receptor population consisted of two functionally distinct receptor populations, designated State 1 and State 2, which were involved in the endocytosis and intracellular processing of ligands by different pathways. The monoclonal antibody 8D7 recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. The monoclonal antibody 8D7 is cross reactive with human ASGPR.
Specifications

Specifications

Catalog number HM3020
Product type Monoclonal antibodies
Quantity 100 µg
Formulation 1 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen Crude rat liver membrane extracts
Isotype Mouse IgG1
Species Rat
Cross reactivity Human - Yes
Alias Asialoglycoprotein receptor 1, ASGPR 1, ASGP-R 1, Hepatic lectin 1, MHL-1
Gene name: Asgr1
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Gastroenterology, Tumor immunology
Application
Applications
  • Application:
    F, FC, IA, IF, W
  • Application Notes:
    W: A non-reduced sample treatment and SDS-Page was used. The band size is 42 kDa (ref.4)
    F: aceton fixed tissue section were incubated Hybridoma culture supernatant
    IA: microtiter plates were coated with 100 µl 20ug/ml for ELISA
  • Positive Control:
    Rat hepatocytes
  • Negative Control:
    Rat kidney, pancreas, small intestine, colon
  • Use:
    Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
References
References
References:
1. Mizuno, M et al: Development of a monoclonal antibody identifying an antigen which- is segregated to the sinusoidal and lateral plasma membranes of rat hepatocytes. Gastroenterol Japan 1986, 21: 238
2. Mizuno, M et al;- Monoclonal antibodies identifying antigens on distinct domains of rat hepatocytes. Liver 1987, 7: 251
3. Hyodo, I et al;- Distribution of asioalogycoprotein receptor in human hepatocellular carcinoma. Liver 1993, 13: 80
4. Shimada, M et al; A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit. Hepatol Res 2003, 26: 55
5. Hirai, M et al. Development of syngeneic monoclonal anti-idiotype antibodies to mouse monoclonal anti-asialoglycoprotein receptor- antibody. Acta med okayama 2002 56: 135
6. Keyel, P et al. A single common portal for clathrin-mediated endocytosis of distinct cargo governed by cargo-selective adaptors. Mol boil Cell 2006 17:4300
7. Zhou, X et al. Effects of hepatitis B virus S protein on human sperm function. Hum Reprod 2009 1: 1
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