TNF-alpha, Human, mAb 4H31, functional study antibody, low endotoxin

Catalog #: HM2009-FS
Quantity: 0.5 mg

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The antibody reacts with human native and recombinant TNF-alpha as assessed by ELISA. The antibody inhibits the biological activity of human native and recombinant TNF-alpha as determined with L929 cells in a cytotoxicity assay. The antibody cross reacts with rhesus and cynomolgus natural TNF-alpha and lacks crossreactivity with human lymphotoxin.


Catalog number HM2009-FS
Product type Monoclonal antibodies
Quantity 0.5 mg
Formulation 0.5 mg (100 µg/ml) 0.2 µm filtered antibody solution in PBS (exact concentration is indicated on the label)
Isotype Mouse IgG1
Species Human
Cross reactivity Cynomolgus monkey - Yes, Rhesus monkey - Yes
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Autoimmunity, Gastroenterology
  • Application:
    F, FC, FS, IA, IP, W
  • Use:
    For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity in vitro dilutions have to be made according to the amount of TNF-alpha to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
1. Gerspach, J et al; Detection of membrane-bound Tumor Necrosis Factor (TNF): an analysis of TNF-specific reagents. Microsc Res Tech 2000, 50: 243
2. Limb, GA et al; Distribution of TNF-alpha and its reactive vascular adhesion molecules in fibrovascular membranes of proliferative diabetic retinopathy. Br J Ophthalmol 1996, 80: 168
3. Laan van der, N et al; Tumor necrosis factor alpha (TNFalpha) in human skin: a comparison of different antibodies for immunohistochemistry. Arch Dermatol Res 2001, 293: 226
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