TNF-RI, Human, mAb H398, biotinylated

Catalog #: HM2021
Quantity: 50 µg
$301.00

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Description
Details
The monoclonal antibody H398 recognizes the extracellular part of the Tumor Necrosis Factor Receptor type I (TNF-RI) of the membrane-bound as well as the soluble receptor. TNF-RI (~55-60 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha.
TNF-RI signaling is initiated when trimeric TNF-alpha binds TNF-RI receptors. Subsequent TNF-RI trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD), Receptor Interacting Protein (RIP), cellular Inhibitor of Apoptosis Protein 1 (cIAP1), TNF Receptor Associated Factor 2 (TRAF2), and likely TRAF5. Studies with TNF-RI-deficient mice indicate that TNF-RI mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways.
Specifications

Specifications

Catalog number HM2021
Product type Monoclonal antibodies
Quantity 50 µg
Formulation 0.5 ml (100 µg/ml) 0.2 μm filtered protein G purified biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Conjugate Biotin
Immunogen Affinity-purified receptor material from HL60 cells containing ~9 μg actively binding receptor (Ref.1).
Isotype Mouse IgG2a
Species Human
Cross reactivity Rat - Yes
Alias CD120a, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, p55, p60, TNFR-1
Gene name: TNFRSF1A, TNFAR, TNFR1
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
Disease Autoimmunity, Gastroenterology
Application
Applications
  • Application:
    FC, FS, IA, IP, W
  • Application Notes:
    W: Reduced sample conditions were used. A band size of ~50-60 kDa is expected.
    FC: Antibody H398 can be used for extracellular staining.
  • Positive Control:
    THP-1 cells
  • Use:
    Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. The antibody competes with TNF-alpha.
References
References
References:
1. Thoma, B et al; Identification of a 60 kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses. J Exp Med 1990, 172: 1019
2. Grell, M et al; TR60 and TR80 tumor necrosis factor (TNF)-receptors can independently mediate cytolysis. Lymphokine Cytokine Res 1993, 12: 143
3. Scheurich, P et al; Agonistic and antagonistic antibodies as a tool to study the functional role of human tumor necrosis factor receptors. Tumor Necrosis factor 1993, 4: 52
4. Grell, M et al; The type 1 receptor (CD120a) is the high-affinity receptor for soluble tumor necrosis factor. Proc Natl Acad Sci USA 1998, 95: 570
5. Krippner-Heidenreich, A et al; Single-chain TNF, a TNF derivative with enhanced stability and antitumoral activity. J Immunol 2008, 180: 8176
6. Kontermann, R et al; A humanized tumor necrosis factor receptor 1 (TNFR1)-specific antagonistic Antibody for selective inhibition of tumor necrosis factor (TNF) action. J Immunother 2008, 31: 225
7. Kälble, F et al; Selective Blocking of TNF Receptor 1 Attenuates Peritoneal Dialysis Fluid Induced inflammation of the Peritoneum in Mice. PLoSONE 2016, 11: e0163314
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