TNF-alpha, Human, mAb T1, FITC
Catalog #: HM2024F
Quantity: 100 µg
The antibody reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. The antibody inhibits the biological activity of both forms. It does not react with receptor bound TNF-alpha. It can be a useful tool to discriminate between the membrane form of TNF expressed on producer cells and the proteolytically cleaved, soluble TNF-alpha bound to its cognate cell membrane receptors (TNF-RI and TNF-RII). For this purpose we recommend to use this antibody in combination with the anti-TNF-alpha antibody HM2026, which recognizes soluble, membrane and receptor bound TNF-alpha.
|Product type||Monoclonal antibodies|
|Formulation||1 ml (100 μg/ml) 0.2 μm protein G purified FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.|
|Immunogen||BALB/c mice with huTNF, fusion of spleen cells with NSO myeloma cells (ref.1)|
|Alias||Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, C-domain 2, Tumor necrosis factor, soluble form
Gene name: TNFA, TNFSF2
|Storage and stability||Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.|
Application:FC, FS, IA, W
Application Notes:FS: The monoclonal antibody T1 can be used for inhibition of the biological activity of TNF-alpha.
W: A reduced sample treatment and SDS-Page was used. The band size is 17 kDa.
Use:Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
1. Gerspach, J et al; Detection of membrane-bound Tumor Necrosis Factor (TNF): an analysis of TNF-specific reagents. Microsc Res Tech 2000, 50: 243