IP-10, Human, mAb 6D4
Catalog #: HM2030
Quantity: 100 µg
The monoclonal antibody 6D4 recognizes human C-X-C motif chemokine 10 (IP-10), a protein of 98 amino acids. IP-10, also known as CXCL10, functions as ligand for the CXCR3 receptor. IP-10 belongs to the α-chemokine (C-X-C) family, which can be divided in two subfamilies: (1) potent chemoattractants for neutrophils, like IL-8 and (2) potent chemoattractants for lymphocytes, like the IFNɣ inducible protein (IP)-10. IP-10 is produced by a wide variety of cell types ranging from neutrophils, dendritic cells and monocytes to hepatocytes, endothelial cells and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV, encephalitis, cutaneous T cell lymphoma, chronic hepatitis, psoriasis and acute anterior uveitis. Various observations strongly suggest a role for the C-X-C chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis. Furthermore IP-10 is associated with acute rejection processes estimated by the predictive properties of urinary IP-10 expression for the short- and long-term graft function after kidney transplantation.
|Product type||Monoclonal antibodies|
|Formulation||1 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.|
|Immunogen||Recombinant human IP-10|
|Alias||10 kDa interferon gamma-induced protein, CXCL10, Small-inducible cytokine B10 Gene name: CXCL10|
|Storage and stability||Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
Application:F, FC, IA, IF, W
Application Notes:FC: Antibody 6D4 stains human CXCL10. For intracellular staining activated plasmacytoid dendritic cells were fixed with 2% paraformaldehyde and permeabilized with 0.5% saponin. All steps were performed in presence of 0.5 M EDTA to avoid cell aggregation (Ref.3) W: Tissue was homogenized in loading sample buffer and subjected to SDS-PAGE. The band size is ~9 kDa (Ref.4). IHC-F: Tissue sections were fixed 10 minutes in acetone and pretreated with avidin/biotin blocking kit. As positive control chronically inflamed liver was used and as negative control normal liver and isotype matched antibody (Ref.4).
Positive Control:Acitvated plasmacytoid dendritic cells
Negative Control:Normal liver
Use:Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
1. Hamamdzic, D. et al; Reovirus triggers cell type-specific proinflammatory responses dependent on the autocrine action of IFNβ. Am J Physiol Lung Cell Mol Physiol 2001, 280: L18 2. Giustizieri, M et al; Nitric oxide donors suppress chemokine production by keratinocytes in vitro and in vivo. Am J Path 2002, 161: 1409 3. Bendriss-Vermare, N et al; Virus overrides the propensity of human CD40L-activated plasmacytoid dendritic cells to produce Th2 mediators through synergistic induction of IFN-gamma and Th1 chemokine production. J of Leukoc Biol. 2005, 78: 954 4. Curbishley, S et al; CXCR3 activation promotes lymphocyte transendothelial migration across human hepatic endothelium under fluid flow. Am J Pathol 2005, 167: 887 5. Wetzel, M et al; μ-Opioid Induction of Monocyte Chemoattractant Protein-1, RANTES, and IFN-g-Inducible Protein-10 Expression in Human Peripheral Blood Mononuclear Cells. J Immunol 2000, 165: 6519