IGF-1R, Human, mAb 1B1
Catalog #: HM2340
Quantity: 100 µg
Monoclonal antibody HM2340, clone 1B1 recognizes human insulin growth factor 1 receptor. IGF-1R is a heterotetrameric transmembrane tyrosine kinase receptor expressed by many cells like lymphoid and myeloid cells as well as non haematopoietic cells. The receptor consists of two extracellular α subunits and two β subunits with tyrosine kinase activity. Receptor autophosphorylation follows by binding of its ligands insulin-like growth factor 1 and 2. The IGF/IGF-1R pathway plays important and diverse roles in tissue development and function, cell cycle progression, apoptosis and translation of proteins. IGF-1R regulates normal as well as pathological responses opening opportunities for therapeutic intervention. IGF-1R bears structural and functional resemblance to other tyrosine kinase receptors. Upon IGF binding the receptor is activated and autophosphorylated at the tyrosine residues in intracellular subunits leading to signaling via MAPK and PI3K/Akt pathways. Many tumors and transformed cells have altered IFG-1R expression. Recently the receptor came under the attention of roles in immune regulation. Proinflammatory cytokines, of which many share signaling components like Erk MAP kinases, seem to dampen several components of the IGF pathway. Furthermore, IGF regulates diverse aspects of T-cell, B-cell and monocyte function suggesting a role in pathogenesis of diseases where immunity is altered, like rheumatoid arthritis and IBD. Antibody 1B1 recognizes the extracellular domain if IGF-1R.
|Product type||Monoclonal antibodies|
|Formulation||1 ml (100 μg/ml) 0.2 μm protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.|
|Application||FC, IA, IF|
|Application Notes||IA: HM2340 can be used as a detection antibody. IF: The cells were incubated with anti-IGF-1R MAb 1B1 for 60 min at room temperature and then fixed with 3.7% formaldehyde in PBS for 15 min at room temperature, followed by detection with Alexa 488-conjugated anti-mouse IgG (ref.1).|
|Immunogen||Recombinant IGF-1R (aa 1-932)|
|Alias||Insulin-like growth factor 1 receptor, IGF-I receptor, IGF-1R, CD221 Gene name: IGF1R|
|Storage and stability||Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
|Disease||Autoimmunity, Tumor immunology|
Use:Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
1. Nakadate, Y et al; Generation of a Monoclonal Antibody Against the Extracellular Domain of IGF-1R. Monoclonal antibodies in immunodiagnosis and immunotherapy, 2014, 2:126