Caspase-3, Human, pAb
Catalog #: HP9060
Quantity: 100 µg
The caspase-3 antibody recognizes caspase-3, a member of the peptidase C14A family of enzymes. It is widely expressed and is an integral component of the apoptotic cascade. Caspase-3 is considered to be the major executioner caspase; that is, the primary downstream mediator of apoptotic associated proteolysis. Human pro-caspase-3 is a 32 kDa, 277 amino acid (aa) protein. Normally, it is an inactive, cytosolic homodimer, but following an upstream signal that activates processing proteases, pro-caspase-3 undergoes proteolytic cleavage. Multiple proteases can use caspase 3 as a substrate including caspase-6, -8, and -10, granzyme B, and caspase-3 itself. Active caspase-3 is known to utilize a Cys residue to cleave multiple substrates, including PARP, proIL16, PKCγ & δ, pro-caspases-6, -7 and -9, and βcatenin. Caspase-3 the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. This caspase-3 polyclonal antibody detects endogenous levels of caspase-3 protein.
|Product type||Polyclonal antibodies|
|Formulation||1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.|
|Application||IA, IF, W|
|Application Notes||W: A reduced and non-reduced sample treatment and SDS-Page was used. The band size is ~17 kDa.|
|Immunogen||Synthesized peptide derived from human Caspase-3 around the non-phosphorylation site of S150|
|Cross reactivity||Mouse - Yes, Rat - Yes|
|Alias||CASP-3, Apopain, Cysteine protease CPP32, CCP-32, Protein Yama, SREBP cleavage activity 1, SCA-1 Gene name: CASP3, CCP32|
|Storage and stability||Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
Use:Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.