Caspase-8, Human, pAb
Catalog #: HP9061
Quantity: 100 µg
The caspase-8 antibody recognizes caspase-8, member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. The caspase-8 polyclonal antibody recognizes endogenous caspase-8.
|Product type||Polyclonal antibodies|
|Formulation||1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.|
|Immunogen||Synthesized peptide derived from human Caspase-8 around the non-phosphorylation site of S347|
|Cross reactivity||Rat - Yes|
|Alias||CASP-8, Apoptotic protease Mch-5, Apoptotic cysteine protease, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, ICE-like apoptotic protease 5, MACH Gene name: CASP8, MCH5|
|Storage and stability||Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
Application Notes:W: A reduced and non-reduced sample treatment and SDS-Page was used. The band size is ~55 kDa.
Use:Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.