Caveolin-1, Rat, mAb 7C8
Catalog #: HM3014
Quantity: 100 µg
The monoclonal antibody 7C8 recognizes rat caveolin-1, a membrane protein of ~22 kDa. Caveolae are sphingomyelin/cholesterol-rich membrane domains first discovered as membrane invaginations on the surface of endothelial and epithelial cells. Caveolae are present in most cells, but are especially abundant in adipocytes. In addition to caveolins only two major protein components of caveolae were identified, namely the semicarbazide sensitive amine oxidase (SSAO) and the scavenger receptor CD36. Caveolin cycles between the plasma membrane and intracellular compartments via the endocytotic pathway. Caveolin is involved in the rapid intracellular transport of newly synthesized cholesterol from the ER directly to the caveolae. Caveolin plays an important role in multiple signaling pathways, molecular transport and cellular proliferation and differentiation. Caveolin binds to endothelial nitric oxide synthase leading to enzyme inhibition. Furthermore caveolin is a candidate tumor suppressor gene in many tumors. The specific functions of caveolin-1/caveolae are highly cell and context dependent. The monoclonal antibody 7C8 recognizes caveolin-1α as well as caveolin-1β, which are present in many tissues, like aorta, heart, muscle, lung, adipose white, brown and epidydimal fat. The monoclonal antibody 7C8 can be used to immuno-isolate caveolae.
|Product type||Monoclonal antibodies|
|Formulation||1 ml (100 μg/ml) 0.2 μm protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.|
|Cross reactivity||Mouse - Yes|
|Alias||Gene name: Cav1, Cav|
|Storage and stability||Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
|Disease||Cardiology and metabolism, Tumor immunology|
Application:IF, IP, W
Application Notes:W: Reduced sample treatment and SDS-PAGE was used. The band sizes are ~22 kDa (Caveolin-1beta;) and ~25 kDa (caveolin-1) (Ref.1).
Positive Control:Adipocytes (3T3-L1 adipocytes)
Negative Control:Cytoplasmic extracts of adipocytes
Use:Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
1. Souto, R et al; Immunopurification and characterization of rat adipocyte caveolae suggest their dissociation from insulin signaling. J Biol Chem 2003, 278: 18321 2. Liu, L et al; A Critical Role of Cavin (Polymerase I and Transcript Release Factor) in Caveolae Formation and Organization. J Biol Chem 2007, 283:4314 3. Liu, L et al; Cavin-3 Knockout Mice Show that Cavin-3 Is Not Essential for Caveolae Formation, for Maintenance of Body Composition, or for Glucose Tolerance. PLoSONE 2014, 9: e102935