MPO, Rat, mAb 2D4
Catalog #: HM3030
Quantity: 100 µg
The monoclonal antibody 2D4 reacts with rat myeloperoxidase (MPO). MPO is a glycoprotein with an alpha2beta2 heteromultimer expressed in all cells of the myeloid linage. MPO is abundantly present in azurophilic granules of polymorphonuclear neutrophils. It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes part in the defense of the organism through production of hypochlorous acid (HOCl), a potent oxidant. MPO is rapidly released by activated polymorphonuclear neutrophils. Involvement of MPO has been described in numerous diseases such as atherosclerosis, lung cancer, Alzheimer's disease and multiple sclerosis. Autoimmune antibodies to MPO are involved in Wegeners disease. Since the discovery of MPO deficiency, initially regarded as rare and restricted to patients suffering from severe infections, MPO has attracted more clinical attention. In experimental studies antibodies to MPO can be used for various purposes ranging from flow cytometric analysis to detection of polymorphonuclear neutrophils in tissue sections.
|Product type||Monoclonal antibodies|
|Formulation||1 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.|
|Cross reactivity||Mouse - Yes|
|Alias||Myeloperoxidase Gene name: Mpo|
|Storage and stability||Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.|
|Disease||Cardiology and metabolism, Pulmonology|
Application Notes:IA: the antibody can be used as a detection antibody. FC: Cells are fixed with 4% paraformaldehyde and permeabilized with 0.1% saponine.
Use:Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
1. Liu, G et al; Kinase AKT1 Negatively Controls Neutrophil Recruitment and Function in MiceJ Immunol 2013, 191:2680