Ethenoadenosine, mAb 1G4

Catalog #: HM5005
Quantity: 100 µg
$529.00

Original supplier of innate immunity products since 1994.

Our commitment to quality: Read more!

Contact our support team for more product information.

Can't find your application for a product? Request a sample!

Description
Details
The reaction of chloroacetaldehyde (CAA) with DNA results in the formation of ethenoadenosine also known as 1,N6-ethenodeoxy-adenosine or etheno-A. CAA is formed through metabolization of for example vinyl chloride, a well established carcinogen. In RNA, after in vitro activation, etheno-A and etheno-C are the principle products of RNA damage. Etheno derivate formation may be highest in single-stranded DNA-regions. The monoclonal antibody 1G4 reacts with both the ribose and deoxyribose form of the adduct. The antibody is not cross reactive with non-modified DNA or the normal nucleotides.
Specifications

Specifications

Catalog number HM5005
Product type Monoclonal antibodies
Quantity 100 µg
Formulation 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Isotype Mouse IgG2
Species N/A
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Tumor immunology
Application
Applications
  • Application:
    F, FC, IA, IP, W
  • Use:
    For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
References
References
References:
1. Young, T et al; Development of techniques to monitor for exposure to vinyl chloride: monoclonal antibodies to ethenoadenosine and ethenocytidine. Carcinogenesis 1988, 9: 589
2. Krebs, C et al; Flow cytometric and immunoblot assays for cell surface ADP-ribosylation using a monoclonal antibody specific for ethenoadenosine. Anal Biochem 2003, 314: 108
Downloads