TREM-2, Mouse, mAb 6E9, FITC
Catalog #: HM1129F
Quantity: 100 µg
The monoclonal antibody 6E9 recognizes mouse membrane-bound as well as soluble triggering receptor expressed on myeloid cells-2 (TREM-2). TREM-2 is a 26 kDa transmembrane glycoprotein that consists of a single extracellular immunoglobulin-like domain, a transmembrane region with a charged lysine residue and a short cytoplasmic tail. It associates with DNAX-activation protein 12 (DAP12) for signaling and function. TREM-2 is expressed on immature monocyte-derived dendritic cells. After activation by microbial products or tumor necrosis factor (TNF) and TNF-related proteins, dendritic cells downregulate the expression of TREM-2. TREM-2 is also expressed by osteoclasts and microglia, where it is involved in bone modeling and brain function, respectively. Another role of TREM-2 might be promoting the removal of apoptotic cells, organic matrix and macromolecules by microglia. Defects in TREM-2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). TREM-2, like TREM-1, can be cleaved on the membrane to release a soluble form of TREM-2 (sTREM-2). Elevated levels of sTREM-2 in CSF of multiple sclerosis patients have been detected. This elevated level may inhibit the anti-inflammatory function of the membrane-bound receptor suggesting sTREM-2 to be a possible target for future therapies.
|Product type||Monoclonal antibodies|
|Formulation||1 ml (100 μg/ml) 0.2 μm filtered protein G purified FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.|
|Immunogen||Mouse recombinant TREM-2 protein|
|Alias||Triggering receptor expressed on myeloid cells 2, Triggering receptor expressed on monocytes 2, TREM-2
Gene name: Trem2, Trem2a, Trem2b, Trem2c
|Storage and stability||Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
Application Notes:FC: The typical starting working dilution is 2 μg/250000 cells.
Use:Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
1. Koga, T et al; Costimulatory signals mediated by the ITAM motif cooperate with RANKL for bone homeostasis. Nature 2004, 428: 758