Cathepsin G, Human, Peptide
Catalog #: HC2138
Quantity: 50 µg
Cathepsin G (CG) is a serine protease originating from human neutrophils. Serine proteases are a class of proteases with the amino acid serine as the key residue of their enzymatic center, which initiates the proteolytic cleavage of specific target proteins, the protease substrates. CG is, together with neutrophil elastase (NE), and proteinase 3 (PR3), involved in bacterial defense. For instance CG is crucial for resistance against infection with Staphylococcus aureus and contributes to protection against several fungal infections. In addition, CG mediates (together with NE and PR3) regulatory functions that shape the inflammatory response. Once released in the extracellular environment, CG is able to proteolytically modify chemokines such as CXCL5 and CCL15 thereby increasing their chemotactic activity. CG has also been implicated in the processing of pro-form of interleukin-1 to its active form, to degrade mature TNF and inactivate IL-6. Given the prominent role in inflammation, CG is involved in the pathophysiology of several serious human inflammatory diseases, such as chronic obstructive pulmonary disease (COPD), Crohn's disease, rheumatoid arthritis, cystic fibrosis and other conditions clinically manifested by excessive inflammatory reactions.
|Alias||EC 184.108.40.206, chymotrypsin-like proteinase, neutral proteinase, CATG|
|Formulation||Lyophilized product in PBS, containing 50 µg Cathepsin G. Reconstitute the vial by pipetting distilled or de-ionized water or DMSO (Caution: vial is under vacuum).|
|Storage and stability||Lyophilized product should be stored at 4°C. Store stock solution in aliquots at -20°C. Repeated freeze and thaw cycles will cause loss of activity. Lyophilized product is stable for at least one year, reconstituted and stored at -20°C, the product is stable for 6 months.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
Use:For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.