C3, Human, Natural
Catalog #: HC2125
Quantity: 250 µg
The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. Activation products of the complement cascade contain neo-epitopes that are not present in the individual native components. The complement factor C3 consists of an alpha- and a beta-chain. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3 mg/ml. An inherited deficiency of C3 predisposes the person to frequent assaults of bacterial infections. In ulcerative colitis, and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. Proteolysis by certain enzymes results in the cleavage of C3 into C3a and C3b. C3b becomes attached to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f. The monoclonal antibody 11H9 recognizes both intact C3 and its cleaved products C3b, iC3b, C3d and C3dg. These activation products are present in acute as well as chronic inflammatory conditions. In chronic inflammatory condition, primarily the C3dg product resides at the place of inflammation (C3c being cleared). The chronic processing/activation of C3 is taking place at a lower level, which would reduce detection of the C3 fragments C3b, iC3b, and C3c.
|Formulation||0.25 ml of approximately >1 mg/ml purified natural human C3 isolated from healthy blood donors, in
Phosphate buffered saline, pH 7.2.The blood donors have been tested and found negative for various
viruses (see table: “Human blood test results”). The functional activity is indicated on the label.
|Storage and stability||Product should be stored at -70 °C. Repeated freeze and thaw cycles will cause loss of activity. The exact expiry date is indicated on the label. Use C1q protein within 24 hours after thawing and keep on ice. Remainder amounts should be aliquoted and immediately re-freezed for future use. Aliquots should never be thawed more than once. Under recommended storage conditions, product is stable for at least one year.|
|Disease||Infectious diseases, Nephrology|
Application Notes:The biological activity of C3 is defined as the amount of C3 required to yield 50% lysis of sensitized erythrocytes (EA).
Use:For functional studies, in vitro dilutions have to be optimized in user's experimental setting.