MBL-C, Mouse, ELISA kit

Catalog #: HK209-02
Quantity: 2 x 96 det.
$1,050.00

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Description
Details
Mannose Binding Lectin (MBL) also called mannose- or mannan-binding protein (MBP) is a member of the collectins and an important element in innate immunity. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. It forms a complex with C1r/C1s like serine proteases designated MASP that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q-independent. MBL exhibits complement-dependent anti-bacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver and serum of several vertebrate species. Only one form of human MBL has been characterized, while two forms are found in rhesus monkeys, rabbits, rats and mice. The murine forms are known as MBL-A and MBL-C. The MBL-C concentrations in serum are about 6-fold higher compared to that of MBL-A. MBL-A, but not MBL-C was found to be an acute phase protein in casein and LPS-injection models. MBL-C exists in higher oligomeric forms than MBL-A. In plasma of healthy mice MBL-C concentrations range from 16 to 118 µg/ml. In infectious diseases the MBL-C concentration was not found to increase significantly.
Specifications

Specifications

Catalog number HK209-02
Product type Assays
Quantity 2 x 96 det.
Standard range The measurable concentration range is 3.1-200 ng/ml.
Detection level The minimum concentration which can be measured is mouse 3.1 ng/ml mouse MBL-C.
Working volume 100 µl/well
Species Mouse
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Autoimmunity, Gastroenterology
Application
Applications
  • Application:
    The mouse MBL-C ELISA kit is to be used for the in vitro quantitative determination of mouse MBL-C in serum, plasma or cell culture samples.
  • Principle:
    The mouse MBL-C ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured mouse MBL-C. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of citric acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the mouse MBL-C standards (log). The mouse MBL-C concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
References
References
References:
1. Vries de, B et al; The mannose-binding lectin pathway is involved in complement activation in the course of renal ischemia-reperfusion injury. Am J Pathol 2004, 165: 1677
2. Trouw, L et al; Activation of the lectin pathway in murine lupus nephritis. Mol Immunol 2005, 42: 731
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