MPO, Human, ELISA kit

Catalog #: HK324-02
Quantity: 2 x 96 det.
$1,140.00

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Description
Details
Myeloperoxidase (MPO) is a glycoprotein with a alpha2beta2 heteromultimer expressed in all cells of the myeloid linage. MPO is abundantly present in azurophilic granules of polymorphonuclear neutrophils. It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes part in the defense of the organism through production of hypochlorous acid (HOCl), a potent oxidant. MPO is rapidly released by activated polymorphonuclear neutrophils. Involvement of MPO has been described in numerous diseases such as atherosclerosis, lung cancer, Alzheimer's disease and multiple sclerosis. Autoimmune antibodies to MPO are involved in Wegener's disease. Since the discovery of MPO deficiency, initially regarded as rare and restricted to patients suffering from severe infections, MPO has attracted more clinical attention.
The classical MPO assay is an enzymatic assay for activity of MPO. This classical MPO assay is hampered by the presence of inhibitory compounds in tissue homogenates and plasma. In this type of assays spiking often gives unreliable results. The human MPO ELISA is not influenced by inhibitors of the enzyme activity.
Specifications

Specifications

Catalog number HK324-02
Product type Assays
Quantity 2 x 96 det.
Standard range 0.4 to 100 ng/ml
Detection level 0.4 ng/ml
Working volume 100 µl/well
Species Human
Cross reactivity Bovine - No, Horse - No, Mouse - No, Pig - No, Rabbit - No, Rat - No, Sheep - No
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Disease Cardiology and metabolism, Pulmonology
Application
Applications
  • Application:
    The human MPO ELISA kit is to be used for the in vitro quantitative determination of human MPO in plasma, sputum and cell culture supernatant samples.
  • Principle:
    The human MPO ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human MPO. Biotinylated tracer antibody will bind to captured human MPO. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human MPO standards (log). The human MPO concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
References
References
References:
1. Leclercq A et al; Involvement of intraplaque hemorrhage in atherothombosis evolution via neutrophil protease enrichment. J Leukoc Biol 2007, 82: 1420
2. Martin-Ventura J et al ; Low plasma levels of HSP70 in patients with carotid atherosclerosis are associated with increased levels of proteolytic markers of neutrophil activation. Atherosclerosis 2007, 194: 334
3. Puklo, M et al; Analysis of neutrophil-derived antimicrobial peptides in gingival crevicular fluid suggests importance of cathelicidin LL-37 in the innate immune response against periodontogenic bacteria. Oral Microbiol Immunol 2008, 23: 328
4. Haan de, J et al; Postshock intervention with high-lipid enteral nutrition reduces inflammation and tissue damage. Ann Surg 2008, 248: 842
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