gC1qR, Human, mAb 60.11

Catalog #: HM2014
Quantity: 100 µg
$341.00

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Description
Details
The monoclonal antibody 60.11 recognizes a cell membrane C1q binding molecule that recognises the globular heads of C1q. It is also present in plasma and the extracellular matrix. The molecule is an unusually acidic, single chain protein with an apparent molecular weight of 33 kDa. It does not possess a conventional sequence motif compatible with a membrane spanning segment nor a consensus site for a GPI anchor. gC1q-R migrates as a single chain under both reducing and non-reducing conditions, but it behaves as an oligomer on gel-filtration in non-dissociating conditions. Its multimer formation may be a critical process by which the gC1q-R molecule increases its affinity for multivalent ligands such as C1q.
gC1q-R has been shown to inhibit complement activation by preventing the binding of C1q to antibodies, suggesting that the binding site for gC1q-R and the binding site for immune complexes, which are present on the C1q globular ‘heads’, may be located at the same position. gC1q-R is capable of interacting with several proteins involved in blood clotting, namely, thrombin, prothrombin, the heparinbinding form of vitronectin, the ternary complex, vitronectin-thrombin-antithrombin, as well as high-molecular-weight kininogen and Hageman factor. Besides its role in the complement pathway, gC1q-R participates in enhancement of Fc-receptor and CR1-mediated phagocytosis, procoagulant activity on platelets, and chemotactic activity on mast cells, eosinophils, neutrophils, and fibroblasts. gC1q-R is expressed on a wide variety of cells and can serve as a binding site for plasma and microbial proteins. Its antigenic sites may be cryptic on cells in suspension but become exposed when the cells are fixed by bifunctional cross-linkers. Probably it is also expressed on the cell membrane as a tetramer. Crosslinking or activation may thus bring about a tetrameric assembly of gC1q-R followed by a conformational change within the molecule, thereby exposing epitopes which are otherwise hidden. A form of GC1q-R is also found inside the cell. Intracellular gC1q-R has been shown to bind the cytoplasmic tail of the α1B-adrenergic receptor and to PKCμ.
The monoclonal antibody 60.11 is directed against epitopes situated within the NH2-terminal stretch of gC1q-R corresponding to residues 76-93. Clone 60.11 recognizes the putative C1q binding site and reacts with the mature form, but has poor or no reactivity with the truncated form, lacking residues 74- 95.
Specifications

Specifications

Catalog number HM2014
Product type Monoclonal antibodies
Quantity 100 µg
Formulation 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Immunogen Recombinant-gC1q-R corresponding to mature gC1q-R (amino acids 74-282)
Isotype Mouse IgG1
Species Human
Cross reactivity Rat - Yes, Syrian hamster - Yes
Alias Glycoprotein gC1qBP, GC1q-R protein, hyaluronan-binding protein 1, mitochondrial matrix protein p32, p33
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease Cardiology and metabolism, Tumor immunology
Application
Applications
  • Application:
    FC, FS, IA, IP, P, W
  • Use:
    For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
References
References
References:
1. Ghebrehiwet, B et al; Identification of functional domains on gC1Q-R, a cell surface protein that binds to the globular "heads" of C1Q, using monoclonal antibodies and synthetic peptides. Hybridoma 1996, 5: 333
2. Ghebrehiwet, B et al; Evidence that the two C1q binding membrane proteins, gC1q-R and cC1q-R, associate to form a complex. J Immunol 1997, 159: 1429
3. Ghebrehiwet, B et al; gC1q-R/p33, a member of a new class of multifunctional and multicompartmental cellular proteins, is involved in inflammation and infection. Immunol Rev 2001, 180: 65
4. Grace, K et al; Surface expression of complement receptor gC1q-R/p33 is increased on the plasma membrane of human spermatozoa after capacitation. Biol Reprod 2002, 66: 823
5. Peerschke, E et al; gC1qR/p33 blockade reduces Staphylococcus aureus colonization of target tissues in an animal model of infective endocarditis. Infect Immun 2006, 74: 4418
6. Sansonno, D et al; Role of the receptor for the globular domain of C1q protein in the pathogenesis of hepatitis C virau-related cryoglobulin vascular damage. J Immunol 2009, 183: 6013
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