Mincle, Rat, mAb WEN43
Catalog #: HM3041
Quantity: 100 µg
The monoclonal antibody WEN43 recognizes rat macrophage inducible C-type lectin (Mincle). This receptor is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. For instance, Mincle is involved in mediating inflammatory responses to necrotic cells and is a pathogen sensor for Mycobacterium tuberculosis. It is a member of the Dendritic Cell Inhibitory Receptor (DCIR) group of C-type lectin receptors (CLRs), which are expressed mainly by antigen presenting cells and are encoded in a cluster in the natural killer gene complex. CLRs play crucial roles in defense against pathogens and recognize a variety of carbohydrate moieties. Ligand binding to CLRs leads to the activation of NF-kB via a Syk/CARD9 signaling module. Mincle was originally identified as a lipopolysaccharide-inducible protein in macrophages and has been shown to stimulate inflammatory responses to fungi, although the ligands and signaling pathways involved have not been defined. WEN43 does not bind to other receptors in the rat APLEC family.
|Product type||Monoclonal antibodies|
|Formulation||1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.|
|Immunogen||Rat mincle-Fcgamma2b fusion protein|
|Alias||Marcophage inducible C-type lectin, CLR, CLECSF9, CLEC4E|
|Storage and stability||Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
Application Notes:FC: Antibody WEN43 stains the extracellular domain of Mincle transfected 293 cells. WEN43 does not bind to other receptors in the rat APLEC family (Ref.1). FS: Antibody WEN43 can act complementary in the inhibition of phagocytosis (Ref.1).
Positive Control:BW5417 or 293T cells
Use:For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
1. Lobato-Pascual, A et al; Mincle, the receptor for mycobacterial cord factor, forms a functional receptor complex with MCL and FcεRI-γ. Eur J Immunol 2013, 43: 3167