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Catalog # HM5013

CML, Human, mAb CML26

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€125.00 – €430.00

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The monoclonal antibody CML26 recognizes human CML (carboxymethyl-lysine). CML is known to be formed from the oxidation of both carbohydrates and lipids. This makes CML- a biomarker of general oxidative stress. Carboxymethyl-lysine (CML) is a well-characterized glycoxidation product that accumulates in tissues with age, and its rate of accumulation is accelerated in diabetes. Glycoxidation products are a subset of advanced glycation endproducts (AGEs) that are formed by the nonenzymatic glycation and subsequent irreversible oxidation of proteins. Oxidative stress and protein modification have been implicated in the pathogenesis of the chronic complications of diabetes, including nephropathy and atherosclerosis. The accumulation of CML in long-lived tissue such as skin collagen reflects oxidative stress over an extended period of the life-span, and has been shown to be greater in patients with diabetic complications than those without complications.

Application
Frozen sections, Immuno assays, Immuno fluorescence, Paraffin sections, Western blot

Application Notes
P: fixation in 4% formalin; cardiac tissue sections (4 mm) deparaffinised for 10 min in xylene at room temperature, dehydrated by decreasing ethanol. Sections stained with haematoxylin and eosin. Blocking endogenous peroxidase activity with 0.3% hydrogen peroxide in methanol for 30 min. No heating to prevent artificial induction of CML. (Ref 1)
IF: After fixation in 2% phosphate-buffered glutaraldehyde solution the heart tissue was post-fixed in 1% osmium tetroxide. The tissue was dehydrated through a graded series of ethanol. 0.53.0- mm-thick sections were cut with a glass knife.(Ref 1)

Use
For immunohistochemistry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Positive Control
Intramyocardial arteries

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide
Immunogen
CML-KLH
Isotype
Mouse IgG1
Species
Human
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Cardiology and metabolism
Protocol
Protocol Western Blot
59 kb
Protocol SDS Page
57 kb
Protocol Sandwich ELISA
49 kb
Protocol Immuno Fluorescence
39 kb
Protocol IHC-P
43 kb
Protocol IHC-F
36 kb
CoA-TDS HM4000-6000
HM5013
80 kb
HM5013-20UG
80 kb
Safety Data Sheet
SDS - HM5013-100UG
52 kb
SDS - HM5013-20UG
52 kb

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