sTNF-RII, Human, ELISA kit

Catalog #: HK302-02
Quantity: 2 x 96 det.
$725.00

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Description
Details
Tumor Necrosis Factor is a pleiotropic inflammatory cytokine. On almost all cells receptors for TNF have been detected. TNF-Receptors (TNF-R) were originally designated as TNF-binding proteins. These TNF-binding proteins present in urine and plasma were later found to be the extra-cellular part of the membrane bound cell signalling receptor for TNF-alpha and TNF-beta. Two types of TNF-Receptors (molecules belonging to the family of nerve growth receptor, CD27, CD40 etc) have been identified: TNF-RI (p55/p60), sCD120a and TNF-RII (p75/p80), sCD120b. The molecules share substantial identity in their extracellular part although the intra-cellular parts are structurally unrelated. This difference is reflected by the difference in function of the two receptors. Activation of various cell types leads to proteolytic cleavage of TNF-R resulting in soluble (extracellular part) TNF-R. Soluble TNF-R (sTNF-R) are considered to play a physiological role in TNF binding and inactivation, although also other functions such as stabilization of TNF in circulation have been mentioned. Detection of sTNF-R offers a means of investigating activation of the immune system both in vitro and in vivo. In plasma of healthy individuals approx. 1 - 4 ng/ml of sTNF-R of each type are present. The ELISA kit is also useful for quantitation of Rhesus monkey and Baboon sTNF-RII in plasma, serum and tissue culture media.
Specifications

Specifications

Catalog number HK302-02
Product type Assays
Quantity 2 x 96 det.
Standard range 15.6-1,000 pg/ml
Detection level 25 pg/ml
Working volume 100 µl/well
Species Human
Cross reactivity Baboon - Yes, Monkey - Yes
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Autoimmunity
Application
Applications
  • Application:
    The human soluble TNF-Receptor II ELISA kit is to be used for the in vitro quantitative determination of human sTNF-RII in serum, plasma and cell culture supernatant samples.
  • Principle:
    The sTNF-RII ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 4½ hours. The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured sTNF-RII. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of citric acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the sTNF-RII standards (log). The sTNF-RII concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
References
References
References:
1. Aziz, N et al; Stability of plasma levels of cytokines and soluble activation markers in patients with human immunodeficiency virus infection. J Infect Dis 1999, 179: 843
2. Maier, B et al; Delayed elevation of soluble tumor necrosis factor receptors p75 and p55 in cerebrospinal fluid and plasma after traumatic brain injury. Shock 2006, 26: 122
3. Roselló-Lletí, E et al; Inflammatory activation and left ventricular mass in essential hypertension. Am J Hypertens 2009, 22: 444
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