TNF-alpha, Human, ELISA kit

Catalog #: HK307-02
Quantity: 2 x 96 det.

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Tumor necrosis factor alpha (TNF-alpha), a homotrimeric 17 KD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore, TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro-organisms and by various cytokines but it also induces on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the p55 (RI) and p75 (RII) TNF-receptors. The receptors differ strongly in their intra-cellular signaling pathways. The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor cross-linking.


Catalog number HK307-02
Product type Assays
Quantity 2 x 96 det.
Standard range 8-2,000 pg/ml
Detection level 10 pg/ml
Working volume 100 µl/well
Species Human
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Autoimmunity, Gastroenterology
  • Application:
    The human TNF-α ELISA kit is to be used for the in vitro quantitative determination of human TNF-α in serum, plasma, and cell culture supernatant samples.
  • Principle:
    The human TNF-alpha ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured human TNF-alpha. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of citric acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human TNF-alpha standards (log). The human TNF-alpha concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
1. Molnar, L et al; Detection of TNFalpha expression in the bone marrow and determination of TNFalpha production of peripheral blood mononuclear cells in myelodysplastic syndrome. Pathol Oncol Res 2000, 6: 18
2. Younis, A et al; Staphylococcus aureus leucocidin, a virulence factor in bovine mastitis. J Dairy Research 2005, 72: 188
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