MASP-2/MAp19, Human, mAb 6G12, immuno assay antibody

Catalog #: HM2191-IA
Quantity: 0.5 mg
$1,345.00

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Description
Details
The monoclonal antibody 6G12 reacts with human MASP-2 and human MAp19. MASP-2 is a trypsin-like serine protease and plays an important role in the initiation of the MBL complement activation pathway. Three pathways of complement activation have been reported: the antibody-dependent classical pathway, the antibody-independent alternative pathway and the lectin pathway. Activation of each pathway involves formation of serine protease complexes, which results in activation of the central complement component C3. In the lectin pathway, mannose binding-lectin (MBL)-associated serine proteases (MASPs) form complexes with polymeric lectin molecules which are involved in pattern recognition. Upon binding of the recognition molecules to carbohydrates on the surface of micro organisms, MASPs are converted to their active forms and initiate complement activation. Three types of human MASP have been reported. MASP-1, MASP-2 and MASP-3. Mannan-binding lectin (MBL) and ficolins, in complex with MBL-associated serin proteases (MASPs), are capable of activation the complement system, thus mediating the destruction of infectious agents. MASP-2 cleaves C4 and C2 and is crucial for the activation of downstream complement components. MAp19 is an alternative splicing product of the MASP-2 gene. MAp19 comprises the first two domains of MASP-2 followed by an extra sequence of four unique amino acids (EQSL) at its C-terminal. MASP-2 and MAp19 have been reported to bind to MBL in a calcium-dependent manner.
The monoclonal antibody 6G12 reacts with high affinity to the N-terminal end of MASP-2 and Map19.
Specifications

Specifications

Catalog number HM2191-IA
Product type Monoclonal antibodies
Quantity 0.5 mg
Formulation 0.5 mg of 0.2 μm filtered protein G purified antibody solution in PBS (exact concentration is indicated on the label).
Application IA, W
Application Notes IA: the antibody can be used as detection antibody.
W: Both reduced and non-reduced can be performed. The expected band size for MASP-2 is approximately 75 kDa and for Map19 approximately 20 kDa.
Immunogen rMAp19 (Ref.1)
Isotype Rat IgG1
Species Human
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Infectious diseases
Application
Applications
  • Use:
    Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
References
References
References:
1. Moller-Kristensen, M et al; Levels of mannan-binding lectin-associated serine protease-2 in healthy individuals. J Immunol 2003, 282: 159
2. Krarup, A et al; L-ficolin Is a Pattern Recognition Molecule Specific for Acetyl Groups*. JBC 2004, 46: 47513
3. Ameye, L et al; M-ficolin levels are associated with the occurrence of severe infections in patients with haematological cancer undergoing chemotherapy. Clin Exp Imm 2011, 167:303
4. Zacho, R et al; Studies of the Pattern Recognition Molecule H-ficolin. JBC 2012, 11: 8071
5. Damgaard Sandahl, T et al; The lectin pathway of the complement system is downregulated in Crohn's disease patients who respond to anti-TNF-α therapy. J Crohn Col 2013, 8:521
6. Man-Kupisinska, A et al; A New Ligand-Based Method for Purifying Active Human Plasma-Derived Ficolin-3 Complexes Supports the Phenomenon of Crosstalk between Pattern-Recognition Molecules and Immunoglobulins. PLoS ONE 2016, 11: e0156691
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