E-selectin, Human, mAb ENA1, immuno assay antibody

Catalog #: HM4001-IA
Quantity: 0.5 mg
$1,068.00

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Description
Details
ENA1 reacts with E-selectin CD62-E, previous designated the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1). The antibody reacts with human endothelial cells activated with TNF-alpha, IL-1 or endotoxin. The antibody was found to react also with cells transfected with the E-selectin gene. The antibody inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.
Specifications

Specifications

Catalog number HM4001-IA
Product type Monoclonal antibodies
Quantity 0.5 mg
Formulation 0.5 mg of 0.2 μm filtered antibody solution in PBS (exact concentration is indicated on the label).
Isotype Mouse IgG1
Species Human
Cross reactivity Baboon - Yes, Chimpanzee - Yes, Cynomolgus monkey - Yes, Rhesus monkey - Yes
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Cardiology and metabolism, Neurological disorders
Application
Applications
  • Application:
    F, FS, IA, IP
  • Use:
    For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. In vitro cultured cells can be fixed with 1% paraformaldehyde and kept in PBS plus azide before staining. Tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 µg/ml) in tissue culture medium at 37 °C. As negative control it is advised to use a control murine IgGantibody.
References
References
References:
1. Leeuwenberg, JFM et al; Induction of an activation antigen on human endothelial cells in vitro. Eur J Immunol 1989, 19: 715
2. Leeuwenberg, JFM et al; Adhesion of polymorphonuclear cells to human endothelial cells. Adhesionmolecule dependent, and Fc-receptor-mediated adhesion-molecule-independent mechanisms. Clin and Exp Immunol 1990, 81: 496
3. Leeuwenberg, JFM et al; Functional polymorphism of IgG FcRII (CD32) on human neutrophils. Immunology 1990, 71: 301
4. Leeuwenberg, JFM et al; Interferon-gamma regulates the expression of an adhesion molecule ELAM-1 and IL-6 production by human endothelial cells in vitro. J Immunology 1990, 145: 2110
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