Ficolin-1, Human, pAb

Catalog #: HP9039
Quantity: 50 µg
$384.00

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Description
Details
The polyconal antibody recognizes human Ficolin-1, also known as M-ficolin and ficolin/P35-related protein. Ficolins are a family of pattern recognition molecules containing both a collagen-like domain and a fibrinogen-like domain that are capable of activating the complement system, through association with MASPs, on the surface of microorganisms. In human, three Ficolins are known: Ficolin-1 (M-ficolin), Ficolin-2 (L-ficolin), and Ficolin-3 (H-ficolin). Ficolin-1, is mainly expressed by monocytes, granulocytes and lung cells and is present locally in areas of inflammation. However, it was recently also found in serum suggesting that Ficolin-1 also has a role in systemic immunity like Ficolin-2 and Ficolin-3. Functional analysis of Ficolin-1 has demonstrated that it’s ligand is sialic acid present on bacteria since binding was abolished by prior treatment of the bacteria with sialidase. Furthermore, it was shown that Ficolin-1 facilitates the uptake of E. coli by a leukemic monocyte lymphoma cell line, suggesting that it functions as a phagocytic receptor on the surface of circulating monocytes. Low cord blood concentration of Ficolin-1 has been associated with higher necrotizing enterocolitis-associated fatality and two identified SNPs in the Ficolin-1 gene are associated with the development of rheumatoid arthritis. Ficolin-1 has 75.3% amino acid identity with Ficolin-2 and 47.8% identity with Ficolin-3. Its mRNA is present in leukocytes and lung. Like its family members Ficolin-2 and Ficolin-3, Ficolin-1 is associated with MASPs and sMAP and activates the lectin pathway. The antibody to human Ficolin-1 is cross reactive with human Ficolin-2.
Specifications

Specifications

Catalog number HP9039
Product type Polyclonal antibodies
Quantity 50 µg
Formulation 1 ml (50 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Isotype Rabbit Ig
Species Human
Storage and stability Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Cardiology and metabolism, Infectious diseases
Application
Applications
  • Application:
    FC, IF, W
  • Use:
    For flow cytometry, immunofluorescence and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
References
References
References:
1. Liu, Y et al; Human M-ficolin is a secretory protein that activates the lectin complement pathway. J Immunol 2005, 175: 3150
2. Frederiksen, P. et al; M-ficolin, an innate immune defence molecule, binds patterns of acetyl groups and activates complement. J. Immunol. 2005, 62: 462
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