ZO-1, Human, pAb

Catalog #: HP9056
Quantity: 100 µg
$398.00

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Description
Details
The polyclonal antibody recognizes human zona occludens 1 (ZO-1), an ~220 kDa tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes.
ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling.
Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.
The polyclonal antibody was raised against amino acids 432-1150 of human ZO-1 and recognizes both ZO-1 alpha-minus and alpha-plus. It cross reacts with mouse and dog ZO-1.
Specifications

Specifications

Catalog number HP9056
Product type Polyclonal antibodies
Quantity 100 µg
Formulation 1 ml (100 μg/ml) 0.2 μm protein A purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen Recombinant human GST-ZO-1 (aa 1-888)
Isotype Rabbit Ig
Species Human
Cross reactivity Canine - Yes, Mouse - Yes
Alias Tight junction protein 1, TJP1
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Tumor immunology
Application
Applications
  • Application:
    FC, IF
  • Application Notes:
    IF: For Immunofluorescence intracellular staining is required. Cells were fixed with 4% paraformaldehyde and cells were permeabilized with PBS, 0.1% saponin and 0.5% BSA.
    FC: For intracellular staining Huvec cells were permeabilized with buffer containing 0.1% saponin and 0.5% BSA.
  • Use:
    Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
References
References
References:
1. Balda, M et al; Two classes of tight junctions are revealed by ZO-1 isoforms. Am J Physiol 1993, 264: C918.
2. Pelletier, R et al; Differential distribution of the tight-junction-associated protein ZO-1 isoforms α- plus and α-minus in guinea pig Sertoli cells: a possible association with F-actin and G-actin. Biol Reprod 1997, 57: 367.
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