MGO-modified proteins, Human, mAb MGO-1

Name: MGO-modified proteins, Human, mAb MGO-1
SKU: HM5014
Availability: InStock

€133.00 – €456.00

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Description
Application

The monoclonal antibody MGO-1recognizes human MGO modified proteins. Methylglyoxal (MGO) is an endogenous product of glucose metabolism. Increased production and accumulation of methylglyoxal (MGO), as well as increased modification of proteins by glycoxidation, are hallmarks of aging and diabetes. MGO was shown to modify proteins and to contribute to the accumulation of damaged proteins that can be toxic to cells. A number of studies have shown that MGO levels are significantly elevated in patients with Type 2 Diabetes- and correlates well with fasting plasma glucose and hemoglobin A1c (HbA1c) levels. Moreover, increased formation of the MGO is implicated in renal dysfunction and is known to be involved in the development of DN (diabetic nephropathy).

Application

Immuno assays, Paraffin sections, Western blot

Application Notes

IHC-P: Sections were preincubated in 0.01 mol/l citrate, pH 6, 37°C for 10 min. Tissue sections were fixed in 4% formalin and subsequently embedded in paraffin. As positive control atherosclerotic plaques was used (Ref.1).

Use

For immunohistochemistry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Specifications
Downloads

Product type

Monoclonal antibodies

Amount

100 µg, 20 µg

Formulation

0.2 ml (100 µ/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Immunogen

MGO-modified KLH

Isotype

Mouse IgG1

Species

Human

Storage and stability

Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Disease

Cardiology and metabolism

Protocol