MGO for neurodegenerative disorders and aging
New Species Independent Competitive Assay for MGO!
Why measure MGO?
- Methylglyoxal (MGO) is a highly potent glycating metabolite which is produced during glycose, amino acid as well as fatty acid metabolism.
- MGO forms covalent adducts with the side chains of lysine-, cysteine- and arginine-residues, which is classified as an early glycation process and occurs in all tissues and body fluids.
- Subsequent, later-stage reactions result in the formation of advanced glycation end products termed AGEs (Advances Glycation Endproducts).
- The damaging effect of MGO consist of the covalent cross-linking of proteins, or when the modified amino acid is located in the active site of the enzyme or results in a structural rearrangement of the modified protein.
- In order to counteract the damaging effect of MGO our body has evolved several non-enzymatic as well as non-enzymatic defensis such as the glyoxalase system.
- In C. elegans silencing of the glyoxalase system resulted in a decreased lifespan by 40%. In human, increased production and accumulation of methylglyoxal (MGO) are hallmarks of aging and a number of pathological conditions such as neurodegenerative disorders and diabetes.
Specifications of the assay:
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Assay time: 3.5 hours
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Species independent
- Sample types tested: plasma, serum, urine and faeces
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HIT503 standard consists of MGO labelled HSA (µg/ml)
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Detection limit: 7.8µg/ml HSA-MGO equivalents.
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Dynamic range: 7.8 – 500µg/ml HSA-MGO equivalents.
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Sample volume: 125µl/well
Measurement of MGO in plasma samples:
Species independent assay!