Home \ Antibodies \ CD14, Human, mAb 18D11
Catalog # HM2224

CD14, Human, mAb 18D11

120,00333,00

Please choose the quantity of items you wish to add to your cart…

The monoclonal antibody 18D11 recognizes human monocyte marker CD14, a 53 kDa glycoprotein. CD14 is a glycophosphatidylinositol-linked protein, which is part of the LPS receptor complex. CD14 binds lipopolysaccharide (LPS) and as such, acts as a pattern recognition receptor (PRR). CD14 has also been suggested to mediate phagocytosis of bacteria an apoptotic cells. CD14 is involved in the endotoxin mediated release of Tumor Necrosis Factor-alpha by monocytic cells. CD14 is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is present at low density on a subpopulation granulocytes and activated or transformed B-cells. The monoclonal antibody 18D11 reacts with both soluble and membrane CD14. The monoclonal antibody 18D11 is useful for inhibition of biological activity of sCD14, it inhibits LPS induced TNF production. The monoclonal antibody is cross reactive with pig CD14. Together with a C5a neutralizing monoclonal antibody, 18D11 blocks sepsis in a pig model.

Application
Flow cytometry, Functional studies, Immuno assays

Use
For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of human sCD14 to be inactivated.

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 ml (100 µg/ml) 0.2  µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Isotype
Mouse IgG1
Species
Human
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Infectious diseases
Protocol CoA-TDS HM2200 - 2300
HM2224
77 kb

You may be interested in…

Calculate your ELISA data easily

With the ELISA calculator you can easily calculate ELISA data. Assayfit Pro helps to perform curve fitting. The calculator generates advanced reports, fit graph, fit parameters and goodness of fit are shown.

Hycultbiotech_contact us

Would you like some virtual coffee?

Contact us