Home \ Antibodies \ Collectin-10, Human, mAb 4F9D7
Catalog # HM2356

Collectin-10, Human, mAb 4F9D7

120,00462,00

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Antibody 4F9D7 recognizes human Collectin-10 (CL-L1). The complement system is the first line of defense against pathogens and important component of innate as well as adaptive immunity. It consists of three major pathways, the classical, alternative and lectin pathway. Each pathway is activated in a different way. The lectin pathway recognizes foreign carbohydrates structures via MBL, ficolins and collectins. CL-L1, CL-K1 (collectin kidney 1 / Collectin11) and CL-P1 (collectin placenta 1/ Collectin12) are the most recently discovered ones. CL-L1 and CL-K1 are quite homolog and have a classical collectin structure and they are highly conserved in mammals. Both CL-L1 and CL-K1 can bind to carbohydrate ligands and DNA of pathogens or on the membrane of damaged cells. They interact with MASPs and activate in a similar way to MBL and ficolins the lectin pathway. Native Cl-L1 displays a calcium dependent binding pattern. A conformational change in carbohydrate recognition domain (CRD) is indicated when the calcium ion is removed by chelation with EDTA. Collectins share a common multimeric structure leading to varying degrees of oligomerization. The chains consist of a collagen-like region, an alpha helicon neck domain and a CRD. CL-L1 was originally found in hepatocytes but mRNA is also found in placenta, adrenal glands lung, small intestine and prostate. Initially it was thought to exist only as a cytoplasmic protein. Recent studies show that is also present in the circulation, either separately of in heteromeric complex with CL-K1.
The average serum concentration is depending on the study, localisation and health status of the population. It is considered to be approximately 300ng/ml for healthy individuals. CL-L1 is encoded by a gene called COLEC10 on chromosome 8. A few polymorphisms have been identified which can lead to increase (up to 40%) of the CL-L1 serum concentration. In SLE patient CL-L1 levels are decreased by approximately 20%. High levels of CL-L1 are beneficial at time of kidney transplantation and is associated with increased long term overall mortality. Elevated levels have also been found in the early phase of acute liver failure and in cirrhosis. Due to (short) periods of microbial challenge fluctuations in CL-L1 in healthy individuals are seen in a given period. In plasma higher levels of CL-L1 are found than in serum (ca 30%).

Application
Immuno assays, Western blot

Application Notes
IA: Antibody 4F9D7 can be used as a capture antibody.
W: Reduced SDS-Page. Expected band sizes are ~37 kDa and 75 kDa.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 ml (100 μg/ml) 0.2 μm protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype
Mouse IgG1
Species
Human
Alias
Collectin liver protein 1, CL-L1, Collectin-34, CL-34<br> - Gene name: COLEC10, CLL1
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Infectious diseases, Nephrology
CoA-TDS HM2300-HM2400
HM2356
87 kb

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