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Catalog # HM3012

Endothelial cell antigen, Rat, mAb RECA-1


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The monoclonal antibody RECA-1 reacts with Rat Endothelial Cell Antigen (RECA), a cell surface antigen (MCA970R) on rat endothelial cells. Endothelial cells (EC) line the interior of all blood vessels and are the key players in the angiogenesis cascade. EC are the first cells and barrier that vehicles or medicines encounter after systemic delivery. Furthermore, they have a signaling function to the cells of the immune system to indicate the status of the surrounding tissue. RECA-1is at least reactive with the rat MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of the RECA-1 monoclonal antibody was seen with other cell types e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens

Flow cytometry, Frozen sections, Immuno fluorescence, Paraffin sections

Application Notes
FC: Standard procedure
IHC-P: In many cases methyl-Carnoys fixative was used to fix tissue
IHC-F:- Standard procedure

For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
Glomerular endothelial cells, RHEC cell line

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Mouse IgG1
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Neurological disorders

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