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Catalog # HP9058

FHR4, Human, pAb

120,00413,00

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Polyclonal antibody HP9058 recognizes human complement factor H related protein 4 (FHR4). The complement system is the first line of defense against pathogens and facilitates elimination of apoptotic and damaged cells. Complement factor H (fH) is an abundant plasma protein that negatively regulates the alternative pathway and the C3b amplification loop. The proteins encoded by the fH gene family includes besides fH also five fH-related proteins (FHR1-5), encoded by closely linked but unique genes. Like fH these proteins consist of repeating short consensus repeats (SCRs). fH competes with fB for binding to C3b and C3b(H2O) thereby blocking the formation of the convertase, accelerates decay of C3-convertases and is a cofactor for fI. The function of FHR proteins is less clear. FHRs are produced primarily in the liver and secreted in the plasma. FHR4 binds to the C3d region of C3b. In contrast to fH, the FHR4-C3b interaction promotes complement activation. Two transcripts of the FHR4 gene exist: FHR4B and FHR4B, consisting of respectively 9 and 5 SCRs (86& 42kDa). Both variants have different binding profiles to C3b. Suggested ligand in complement activation of FHR4 is pentameric CRP.

Application
Immuno assays, Western blot

Application Notes
W: A non-reduced and/or reduced sample treatment and SDS-Page was used. The band size is 86 kDa.
IA: HP9058 can be used as detection antibody.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Polyclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 ml (100 μg/ml) 0.2 μm protein A purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen
Recombinant FHR4 fragment SCR2-4
Isotype
Rabbit Ig
Species
Human
Alias
Complement factor H-related protein 4, FHR-4 </br> - Gene name: CFHR4, CFHRL4, FHR4
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Autoimmunity, Nephrology
CoA-TDS pAb
HP9058
81 kb

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