Ficolin-3, Human, ELISA kit
Ficolins are a group of proteins containing both a collagen-like domain and a fibrinogen-like domain. Three forms of Ficolin have been identified in humans: Ficolin-1 (M-ficolin), Ficolin-2 (L-ficolin) and Ficolin-3 (H-ficolin 3). Ficolin-3, also known as Hakata-antigen or H-ficolin, is composed by a collagen-like strand and three C-terminal recognition domains which bind to acetyl groups on microbial surfaces such as GlcNAc or GalNAc. Ficolin-3 is synthesized both in the liver, from where it is secreted into the blood circulation, and in the lung. Similar to MBL and Ficolin-2, Ficolin-3 relies on MBL-associated serine protease 2 (MASP-2) for activation of the complement system. After binding of Ficolin-3/MASP-2 complexes to microbial surfaces, MASP-2 sequentially cleaves C4 and C2, thereby generating the C3 convertase C4bC2b, which finally leads to opsonization and direct lysis of pathogens and recruitment of inflammatory cells.
Ficolin-3 is present in serum at mean concentration of 15 µg/ml, with only minor variations. Ficolin-3 was present in all sera from more than 150,000 individuals tested, except in some systemic lupus erythromatosis patients. Approximately 5% of systemic lupus erythromatosis patients were found to be Ficolin-3 negative, probably owing to the presence of anti-Ficolin-3 autoantibody. In 398 patients with other autoimmune diseases, Ficolin-3 was always present. In liver disease the serum levels decreased with increasing severity of cirrhosis.
Ficolin-3 bound to a population of late apoptotic cells, while a strong and uniform binding to necrotic cells was observed. The binding properties differed from those of MBL and Ficolin-2. Ficolin-3 binding to late apoptotic cells resulted in a significant increase in adhesion/uptake by macrophages.
Calculate your ELISA data easily
With the ELISA calculator you can easily calculate ELISA data. Assayfit Pro helps to perform curve fitting. The calculator generates advanced reports, fit graph, fit parameters and goodness of fit are shown.
We are glad to support you!
Our technical support team of qualified scientists is more than willing to assist.