H-FABP, Human, mAb 67D3

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Description
Application

The monoclonal antibody 67D3 recognizes human heart-type fatty acid-binding protein (H-FABP) of both natural and recombinant origin. The H-FABP protein is derived from the human FABP3 gene. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity that bind long chain fatty acids. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. H-FABP is localized in the heart, skeletal and smooth muscle, mammary epithelial cells, aorta, distal tubules of the kidney, lung, brain, placenta, and ovary. Furthermore, this antibody is useful for the purification of H-FABP.

Application

Frozen sections, Immuno assays, Immuno precipitation, Western blot

Application Notes

IP: Biotinylated 67D3 was immobilized on streptavidin beads and added to serum to immunoprecipitate H-FABP (Ref.3).
W: Reduced sample treatment. The band size is ~15 kDa (Ref.2).
F: Permeabilization was done in cold acetone with 0.5% hydrogen peroxidase for 10 min, after drying and washing, antibodies were incubated for 30 minutes.

Use

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control

Heart cells, recombinant human H-FABP

Negative Control

Tonsil tissue

Specifications
Downloads

Product type

Monoclonal antibodies

Amount

100 µg, 20 µg, 500 µg

Formulation

Quantities >500 µg will be send without preservative and carrier free. Quantities <500 µg will be send with preservative and carrier. exact formulation can be found on the datasheet.

Immunogen

Purified Human H-FABP

Isotype

Mouse IgG1

Species

Human

Cross reactivity

Mouse – Yes, Rat – Yes

Alias

Heart-type fatty acid-binding protein, Fatty acid-binding protein 3, FABP3, Muscle fatty acid-binding protein, Mammary-derived growth inhibitor<br> - Gene name: FABP3, FABP11, MDGI

Storage and stability

Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.

Disease

Cardiology and metabolism, Neurological disorders

Protocol