Halotyrosine, clone BTK-94C is recommended for detection of bromotyrosine modified proteins. Halogenation is a chemical reaction that substitutes a molecule’s hydrogen atom with halogen, a nonmetal element. Fluorination, chlorination, bromination and iodination are the four types of halogenation. Halogenated organic compounds are found as natural products in many living organisms. Halotyrosine residues are the result of tyrosine modification, usually bromine or chlorine. This generally occurs as a result of immune cell actions or oxidative stress. For example, activated eosinophils release eosinophil peroxidase, which in turn produces hypobromite (HOBr). Hypobromite can then react with proteins to create bromotyrosine residues. Studies on total bromotyrosine levels have shown that these protein modifications are increased in asthmatics, but are decreased in response to anti-inflammatory drugs.
Immuno assays, Immuno fluorescence, Immuno precipitation, Western blot
W: A reduced sample treatment was used. The band size is approximately 33 kDa.
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
SK-MEL-28 cell lysate