Halotyrosine, mAb BTK-94C

Name: Halotyrosine, mAb BTK-94C
SKU: HM5016
Availability: InStock

Halotyrosine, clone BTK-94C is recommended for detection of bromotyrosine modified proteins and suitable for immuno assays, Immuno fluorescence, Immuno precipitation, Western blot applications.

€139.00 €474.00Price range: €139.00 through €474.00

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Description
Application

Halogenation is a chemical reaction that substitutes a molecule’s hydrogen atom with halogen, a nonmetal element. Fluorination, chlorination, bromination and iodination are the four types of halogenation. Halogenated organic compounds are found as natural products in many living organisms. Halotyrosine residues are the result of tyrosine modification, usually bromine or chlorine. This generally occurs as a result of immune cell actions or oxidative stress. For example, activated eosinophils release eosinophil peroxidase, which in turn produces hypobromite (HOBr). Hypobromite can then react with proteins to create bromotyrosine residues. Studies on total bromotyrosine levels have shown that these protein modifications are increased in asthmatics, but are decreased in response to anti-inflammatory drugs.

Application

Immuno assays, Immuno fluorescence, Immuno precipitation, Western blot

Application Notes

W: A reduced sample treatment was used. The band size is approximately 33 kDa.

Use

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control

SK-MEL-28 cell lysate

Specifications
Downloads

Product type

Monoclonal antibodies

Amount

100 µg, 20 µg

Formulation

0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Immunogen

Bromotyrosine structural mimic conjugated to KLH

Isotype

Mouse IgM

Species

N/A

Storage and stability

Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Disease

Cardiology and metabolism, Gastroenterology, Pulmonology

CoA-TDS HM4000-6000