Home \ Proteins and peptides \ C9, Human, Natural
C9, Human, Natural-0
Catalog # HC2142-250UG

C9, Human, Natural

Read more
Please choose the quantity of items you wish to add to your cart…
Find distributor

Glycoprotein complement factor C9 is synthesized as a single chain protein of 558AA and is present in human serum at ca 60ug/ml. C9 is predominantly formed in the liver, but is also synthesized by monocytes, macrophages, fibroblasts and glial cells. Pore forming proteins with a membrane-attack complex perforin domain (MACPF) are most effective to kill bacteria or virus infected cells. The MACPF domain polymerizes, refolds and inserts itself into the bilayer membrane. C5b binds to the membrane and binds C6&7. The addition of C8 to the complex triggers and accelerates C9 polymerization in order to form the asymmetric pore on the target surface. A complete pore can exist out of 12-18 C9 monomers. Together with CD59, MAC formation is regulated by eg DAF, MCP and CR1. C9 deficiency is quite common, especially in the Japanese population. These patients are more susceptible for bacterial infection.

Application
Functional studies

Product type
Proteins
Amount
250 µg
Species
Human
Formulation
0.25 ml of approximately 1 mg/ml purified natural human C9 isolated from healthy blood donors, in Phosphate buffered saline, pH 7.2. The blood donors have been tested and found negative for various viruses (see table: Human blood test results).
Storage and stability
Product should be stored at -70 °C. Repeated freeze and thaw cycles will cause loss of activity. The exact expiry date is indicated on the label. Use C9 protein within 24 hours after thawing and keep on ice. Remainder amounts should be aliquoted and immediately re-freezed for future use. Aliquots should never be thawed more than once. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Infectious diseases, Nephrology
CoA-TDS Protein
HC2142
67 kb
Safety Data Sheet
HC2142-250UG
50 kb

You may be interested in…

Calculate your ELISA data easily

With the ELISA calculator you can easily calculate ELISA data. Assayfit Pro helps to perform curve fitting. The calculator generates advanced reports, fit graph, fit parameters and goodness of fit are shown.

Show data entry

Latest Hycult Biotech news

  • New Human Complement Pathway Assays
    We are very proud of our newly developed human classical and alternative complement pathway assays. They are produced in response to a growing demand for quantitative investigation of complement inhibitors or regulators at lower sample dilutions. This development aims to address the issue of false negative results, enabling more accurate and reliable analysis of complement… Read more: New Human Complement Pathway Assays
  • Navigating the pitfalls in complement analysis
    Our colleague Erik Toonen shared his experience on how to analyze complement at the Complement-based Drug Development Summit in Boston in September 11-13th, 2023. He showed valuable insights on analyzing complements. For accurate complement analysis, it is important that not only the correct technique is used but also that pre-analytical sample handling is performed in… Read more: Navigating the pitfalls in complement analysis
  • NEW Human C3d ELISA
    We are happy to introduce our new ELISA (cat.# HK3017) designed to detect human C3d. What sets this assay apart is its utilization of a distinctive neo-epitope capture antibody that selectively targets a specific C3d region of the alpha chain, which is not available in other C3 variants. This way it specifically distinguishes C3d from native C3… Read more: NEW Human C3d ELISA
Contact us