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Catalog # HM1051BT-50UG

MPO, Mouse, mAb 8F4, biotinylated

The monoclonal antibody 8F4 recognizes mouse myeloperoxidase (MPO).

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The monoclonal antibody 8F4 recognizes mouse myeloperoxidase (MPO).

MPO is a glycoprotein produced as a single precursor, which is subsequently cleaved into a alpha and beta chain. In human the biologically active MPO is a 150kDa tetramer composed of 2 glycosylated alfa chains of 59-64 kDa and 2 beta chains of 14 kDa. MPO is stored in azurophilic granules of polymorphonuclear leukocytes and is rapidly released into the phagosome and extracellular space during inflammatory conditions.The enzyme catalyzes the conversion of chloride and hydrogen peroxide to hypochlorite, a potent oxidant, which functions in host defense against microorganisms.

Involvement of MPO has been described in several human diseases, such as cardiovascular disease, airway inflammation, lung cancer, Alzheimer’s disease and multiple sclerosis. A positive correlation between elevated MPO levels in serum and cardiovascular disease suggest an interesting role for MPO as an diagnostic marker, making it possible to identify patients at risk for future cardiac events. Furthermore, there are some autoimmune diseases, in which MPO is targeted by antineutrophil cytoplasm antibodies. Studies with MPO-knockout mice have shown an increased susceptibility to pneumonia following intratracheal infections. Moreover, MPO deficient mice are more susceptible to experimental autoimmune encephalitis, a T cell-dependent neuronal disease, and have an increased expression of arteriosclerotic plaques compared to wild-type mice.

The anti-mouse MPO monoclonal antibody 8F4 recognizes natural MPO in biological solutions by ELISA, in frozen tissue sections fixed with acetone and in flow cytometry using a cell permeabilization method.

Flow cytometry, Frozen sections, Immuno assays, Immuno fluorescence

Application Notes
IA: the antibody can be used as a detection antibody.
FC: Cells are fixed with 4% paraformaldehyde and permeabilized with 0.1% saponin.
IA: HM1051 was used as a capture antibody. The experiment performed was a specificity experiment with different concentrations of HM1051.

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
Neutrophils isolated from digested infarcts

Negative Control
Lymphocytes isolated from digested infarcts

Product type
Monoclonal antibodies
50 µg
0.5 ml (100 μg/ml) 0.2 μm filtered protein G purified biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Purified mouse MPO from WEHI-3 cells
Mouse IgG1
Myeloperoxidase</br> - Gene name: Mpo
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Cardiology and metabolism, Pulmonology
Protocol CoA-TDS HM1000-HM1066 Safety Data Sheet

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