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Catalog # HM2003

IFN-gamma, Human, mAb F12

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Monoclonal antibody binds and neutralizes both natural and recombinant human gamma Interferon. Cross reactivity with other cytokines has not been found. The antibody does not react with rodent interferons. Possible applications include the purification of human gamma Interferon and in vitro neutralisation. In general, monoclonal antibodies to gamma Interferon are able to inhibit Schwartzman reactions and in the murine system appear to protect NZB mice against spontaneous development of autoimmune disease.

Application
Frozen sections, Functional studies, Immuno assays, Immuno precipitation

Use
For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity in vitro dilutions have to be made according to the amount of IFN-gamma to be inactivated. One neutralizing unit of anti-human gamma interferon is defined as the amount of antibody sufficient for neutralizing one unit human gamma interferon (ref.: NIH standard Gg23-901-530).

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg, 300 µg
Formulation
Lyophilized product in PBS, containing 100 µg antibody. Reconstitute the vial by injection of 1 ml distilled or de-ionized water (Caution: vial is under vacuum).
Isotype
Mouse IgG1
Species
Human
Storage and stability
Lyophilized product should be stored at 4 °C. Store stock solution in aliquots at -20 °C. Repeated freeze and thaw cycles will cause loss of activity. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Autoimmunity, Infectious diseases
Protocol
Protocol Sandwich ELISA
49 kb
Protocol Immuno precipitation
54 kb
Protocol IHC-F
36 kb
CoA-TDS HM1067-HM2099
HM2003-100UG
52 kb
HM2003-20UG
53 kb
HM2003-300UG
53 kb
Safety Data Sheet
SDS - HM2003-100UG
51 kb
SDS - HM2003-20UG
51 kb
SDS - HM2003-300UG
51 kb

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