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Catalog # HM1002

IFN-gamma, Mouse, mAb F3

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Monoclonal antibody F3 binds and neutralizes both natural and recombinant mouse gamma Interferon. Its binding and neutralizing activity has been demonstrated in vitro and in vivo. F3 antibodies have been demonstrated to be able to inhibit inflammatory responses to bacterial lipopolysaccharides. These antibodies were furthermore shown to inhibit Shwartzman reactions and to protect NZB mice against spontaneous development of autoimmune disease. The antibody does not react with rat or human gamma interferon.

Application
Functional studies, Immuno assays, Immuno precipitation

Application Notes
FS: The antibody can be used for in vitro and in vivo inhibition of activity of IFN-gamma. For neutralisation of biological activity dilutions have to be made according to the amount of Interferon to be inactivated. One neutralizing unit is defined as the amount of antibody sufficient for neutralizing one unit of mouse gamma Interferon antiviral activity as calibrated against NIH standard Gg02-901-533.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with HIT302 LAL Assay.
Isotype
Rat IgG2a
Species
Mouse
Alias
IFN-γ
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Autoimmunity, Infectious diseases
Protocol
Protocol Sandwich ELISA
49 kb
Protocol Immuno precipitation
54 kb
CoA-TDS HM1000-HM1066
HM1002
82 kb
HM1002-500UG
73 kb
HM1002-FS
82 kb
Safety Data Sheet
SDS - HM1002-100UG
51 kb
SDS - HM1002-20UG
51 kb
SDS - HM1002-500UG
50 kb

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