The antibody neutralizes mouse interleukin 6 (IL-6). It does not cross-react with LPS (the component of the
bacterial cell wall which is considered responsible for the toxicity of gram-negative bacteria) and TNFalpha
nor does it block human or rat IL-6 in proliferation assays using the KD83 cell line. MP5-32C11
inhibits mouse IL-6-induced proliferation of the NFS60 myelomonocytic cell line and KD83 plasmacytoma.
IL-6 is a pluripotent 20-22 kDa cytokine which plays a role in the pathophysiology of severe infection and
regulates the immune response, acute phase reaction and haematopoiesis. IL-6 plays a critical role in Bcell
differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma.
Continuous IL-6 gene expression makes an essential contribution to a multistep oncogenesis of plasma
cell neoplasia. IL-6 is a very useful culture supplement for the generation of a high number of antibodyproducing
Functional studies, Immuno assays, Immuno precipitation, Western blot
The antibody can be used for immunochemical investigations of mouse IL-6 in structural studies and for
quantitative/qualitative immunochemical detection methods. The antibody neutralizes the biological activity
of mouse IL-6. Furthermore the antibody is useful for Western blot and as tracer antibody use in immuno
For Western blotting dilutions to be used depend on detection system applied. It is recommended that
users test the reagent and determine their own optimal dilutions. The typical starting working dilution is
1:10. For neutralisation of biological activity dilutions have to be made according to the amount of IL-6 to be